Multipurpose membranes, methods for forming, and applications thereof

Patent metadata

Field	Value
Publication number	US-10086110-B2
Application number	US-201314067120-A
Country	US
Kind code	B2
Filing date	Oct 30, 2013
Priority date	Oct 30, 2012
Publication date	Oct 2, 2018
Grant date	Oct 2, 2018

Abstract

One aspect of the present disclosure relates to a method for forming a multipurpose membrane in vivo. One step of the method includes obtaining a blood component. Next, a vacuum assembly is operated to remove substantially all of the liquid from the blood component and thereby form a concentrated, substantially dehydrated blood component. The substantially dehydrated blood component is then formed into a non-coagulated injectable composition and administered to a wound of a subject.

First claim

The following is claimed:

1. An ex vivo method for forming a multipurpose membrane, the method comprising the steps of:
obtaining a volume of whole blood;
contacting the volume of whole blood with an amount of thrombin and a source of calcium ions to form a coagulated whole blood component;
placing the coagulated whole blood component in a wicking assembly;
removing a liquid from a first surface of the coagulated whole blood component; and
removing a liquid from an oppositely disposed, different second surface of the coagulated whole blood component;
wherein removing liquid from the first and second surfaces results in the coagulated whole blood component having a water content of less than about 10%.

2. The method of claim 1 , wherein the coagulated whole blood component is autologous, allogeneic or xenogeneic.

3. The method of claim 1 , wherein the whole blood is subjected to ultrafiltration before contacting the whole blood with the thrombin and the source of calcium ions.

4. The method of claim 1 , wherein the step of operating a vacuum assembly further comprises the steps of:
providing a vacuum assembly comprising a housing having a receiving portion in fluid communication with a suction mechanism;
disposing the coagulated whole blood component in the receiving portion; and
operating the suction mechanism to generate a negative pressure within the receiving portion and thereby remove substantially all of the liquid from the coagulated whole blood component.

5. The method of claim 1 , wherein the placing step further comprises pouring the coagulated white blood component into a mold.

6. The method of claim 1 , being performed at about room temperature.

7. The method of claim 1 , wherein the membrane is formed in less than about 30 minutes.

8. The method of claim 1 , further including the step of imbibing the membrane with one or more therapeutic agents.

9. The method of claim 1 , wherein the coagulated whole blood component is fully coagulated after the contacting step.

10. The method of claim 1 , wherein only operation of the wicking assembly is performed to remove the liquid from the coagulated whole blood component.

11. An ex vivo method for forming a multipurpose membrane, the method consisting of:
obtaining a volume of whole blood;
contacting the volume of whole blood with an amount of thrombin and a source of calcium ions to form a coagulated whole blood component;
placing the coagulated whole blood component in a wicking assembly;
removing a liquid from a first surface of the coagulated whole blood component; and
removing a liquid from an oppositely disposed, different second surface of the coagulated whole blood component;
wherein removing liquid from the first and second surfaces results in the coagulated whole blood component having a water content of less than about 10%.

Assignees

• Cleveland Clinic Found

Inventors

• Evans Peter J
• Trickett Jim

Classifications

• A61L2430/32

  for nerve reconstruction · CPC title

• A61L26/0042

  Fibrin; Fibrinogen · CPC title

• A61L26/0057 Primary

  Ingredients of undetermined constitution or reaction products thereof · CPC title

• A61L24/0005

  Ingredients of undetermined constitution or reaction products thereof · CPC title

• A61L27/3616

  Blood, e.g. platelet-rich plasma · CPC title