Compositions and method for light triggered release of materials from nanovesicles

Patent metadata

Field	Value
Publication number	US-10086074-B2
Application number	US-201314654085-A
Country	US
Kind code	B2
Filing date	Dec 19, 2013
Priority date	Dec 19, 2012
Publication date	Oct 2, 2018
Grant date	Oct 2, 2018

Abstract

Provided are compositions comprising porphyrin-phospholipid nanovesicles (PoP-NVs) which can be loaded with cargo. Methods for release of cargo from the PoP-NVs triggered by near infrared (NIR) light are also disclosed.

First claim

The invention claimed is:

1. A composition comprising nanovesicles, said nanovesicles comprising a bilayer which comprises a porphyrin conjugate having the following structure:

wherein m is an integer from 1 to 12, n is an integer from 1 to 12, and R 1 is a branched or liner alkyl group having from 2 to 20 carbon atoms, wherein the nanovesicle bilayer comprises 0.1 to 14.4 mol. % of the porphyrin conjugate.

2. The composition of claim 1 , wherein the porphyrin conjugate is selected from one of the following structures:

3. The composition of claim 1 , wherein the nanovesicles have an average size of 30 to 250 nm.

4. The composition of claim 3 , wherein the nanovesicles have an average size of 100 to 175 nm.

5. The composition of claim 1 , wherein the nanovesicle bilayer comprises 9.5 to 10.5 mol. % porphyrin conjugate.

6. The composition of claim 1 , wherein the bilayer further comprises one or more of distearoyl phosphatidylethanolamine (DSPE), DSPE-polyethylene glycol (DSPE-PEG), or cholesterol.

7. The composition of claim 1 , wherein the nanovesicles are loaded with cargo, said nanovesicles being releasable after exposure of the nanovesicles to electromagnetic radiation of from 650 to 1000 nm.

8. The composition of claim 7 , wherein the cargo comprises a therapeutic agent or a diagnostic agent.

9. The composition of claim 7 , wherein the cargo comprises doxorubicin.

10. A method of delivering cargo to a desired location comprising the steps of:
a) administering to an individual the composition of claim 7 such that it enters the circulatory system;
b) allowing the nanovesicles to reach the desired location;
c) exposing the nanovesicles to near infrared radiation of wavelength from 650 to 1000 nm such that the cargo is released from the nanovesicles.

11. The method of claim 10 , wherein the composition is administered to the individual via intravenous route.

12. The method of claim 10 , wherein the individual is a human or non-human mammal.

13. The method of claim 10 , wherein the nanovesicles are exposed to a wavelength of 658, 665, or 671 nm.

14. The method of claim 10 , wherein the nanovesicles are exposed to the near infrared radiation for up to 30 minutes.

15. The method of claim 10 , wherein step c) is carried out as multiple exposures to the near infrared radiation.

Assignees

• Univ New York State Res Found
• Health Research Inc

Inventors

• Lovell Jonathan
• Pandey Ravindra
• Carter Kevin
• Shao Shuai

Classifications

• C07D487/22

  in which the condensed system contains four or more hetero rings · CPC title

• A61K31/7048

  having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin {, digitoxin or digoxin} · CPC title

• A61K9/1271

  Non-conventional liposomes, e.g. PEGylated liposomes or liposomes coated or grafted with polymers (liposomes as conjugates {A61K47/6911}) · CPC title

• A61K9/127

  Synthetic bilayered vehicles, e.g. liposomes or liposomes with cholesterol as the only non-phosphatidyl surfactant · CPC title

• A61K31/704

  attached to a condensed carbocyclic ring system, e.g. sennosides, thiocolchicosides, escin, daunorubicin {(digitoxin A61K31/7048)} · CPC title