Thraustochytrids, fatty acid compositions, and methods of making and uses thereof

Patent metadata

Field	Value
Publication number	US-10085465-B2
Application number	US-201615209568-A
Country	US
Kind code	B2
Filing date	Jul 13, 2016
Priority date	Mar 19, 2009
Publication date	Oct 2, 2018
Grant date	Oct 2, 2018

Abstract

The present invention is directed to isolated thraustochytrid microorganisms as well as strains and mutants thereof. The invention is further directed to biomasses, microbial oils, compositions, cultures, methods of producing microbial oils, and methods of using the isolated thraustochytrids, biomasses, and microbial oils.

First claim

What is claimed:

1. An isolated thraustochytrid biomass, wherein at least 50% by weight of the dry cell weight of the biomass are fatty acids, and wherein at least 50% by weight of the fatty acids are omega-3 fatty acids.

2. The isolated thraustochytrid biomass of claim 1 , wherein at least 50% by weight of the fatty acids is docosahexaenoic acid.

3. An isolated thraustochytrid biomass, wherein at least 25% by weight of the dry cell weight of the biomass is docosahexaenoic acid.

4. The isolated thraustochytrid biomass of claim 1 or claim 3 , wherein 10% or less by weight of the fatty acids is eicosapentaenoic acid, and wherein the weight ratio of docosahexaenoic acid to eicosapentaenoic acid is at least 5:1.

5. The isolated thraustochytrid biomass of claim 4 , wherein 1.5% or less by weight of the fatty acids is arachidonic acid, and wherein the weight ratio of docosahexaenoic acid to arachidonic acid is at least 20:1.

6. The isolated thraustochytrid biomass of claim 5 , further comprising docosahexaenoic acid and docosapentaenoic acid n-6 in a weight ratio of at least 10:1.

7. A food product, cosmetic, or pharmaceutical composition for animals or humans, comprising the thraustochytrid biomass of claim 2 or claim 4 .

8. The food product of claim 7 , wherein the food product is an additive for animal or human food.

9. The food product of claim 7 , wherein the food product is a nutritional supplement.

10. The food product of claim 7 , wherein the food product is an animal feed.

11. The animal feed of claim 10 , wherein the animal feed is an aquaculture feed.

12. The animal feed of claim 10 , wherein the animal feed is a domestic animal feed, a zoological animal feed, a work animal feed, a livestock feed, or a combination thereof.

13. The isolated thraustochytrid biomass of claim 2 or claim 4 , wherein said biomass further comprising at least one antioxidant.

14. The food product of claim 7 , wherein said food product further comprising at least one antioxidant.

15. The animal feed of claim 11 , wherein said animal feed further comprising at least one antioxidant.

16. The isolated thraustochytrid biomass of claim 13 , wherein said at least one antioxidant is in an amount that is sufficient to inhibit the oxidation of the biomass.

17. The isolated thraustochytrid biomass of claim 1 , wherein at least 60% by weight of the fatty acids are omega-3 fatty acids.

18. The isolated thraustochytrid biomass of claim 1 , wherein at least 55% by weight of the fatty acids is docosahexaenoic acid.

19. The isolated thraustochytrid biomass of claim 1 , wherein at least 30% by weight of the dry cell weight of the biomass is docosahexaenoic acid.

20. A feed product for animals, comprising the thraustochytrid biomass of claim 17 , wherein said animal feed further comprises at least one antioxidant, wherein said at least one antioxidant is in an amount that is sufficient to inhibit the oxidation of the biomass.

21. A teed product for animals, comprising the thraustochytrid biomass of claim 18 , wherein said animal feed further comprises at least one antioxidant, wherein said at least one antioxidant is in an amount that is sufficient to inhibit the oxidation of the biomass.

22. A feed product for animals, comprising the thraustochytrid biomass of claim 19 , wherein said animal feed further comprises at least one antioxidant, wherein said at least one antioxidant is in an amount that is sufficient to inhibit the oxidation of the biomass.

Assignees

• Dsm Ip Assets Bv

Inventors

• Apt Kirk E
• Pfeifer Iii Joseph W
• Hansen Jon Milton
• Behrens Paul Warren
• Zirkle Ross
• Stahl Tracey Lynn

Classifications

• A61P29/00

  Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID] · CPC title

• A23D9/00

  Other edible oils or fats, e.g. shortenings or cooking oils (preservation of other edible oils A23B20/30) · CPC title

• A23K20/158 Primary

  Fatty acids; Fats; Products containing oils or fats · CPC title

• A61K2035/11

  Medicinal preparations comprising living procariotic cells · CPC title

• A23L33/135

  Bacteria or derivatives thereof, e.g. probiotics · CPC title