Barcode sequences, and related systems and methods
US-2016333402-A1 · Nov 17, 2016 · US
Delaying real-time sequencing
US10081836B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10081836-B2 |
| Application number | US-201615211890-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 15, 2016 |
| Priority date | Oct 24, 2013 |
| Publication date | Sep 25, 2018 |
| Grant date | Sep 25, 2018 |
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- Title
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Abstract
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Methods, compositions, and systems are provided that allow for reliable sequencing of the initial sequence region of a sequence of interest. The methods of the invention allow for more reliable barcoding of subpopulations of nucleic acids to be sequenced.
First claim
Opening claim text (preview).
What is claimed is: 1. A method for delaying the sequencing of a sequence of interest in a single-molecule, real-time sequencing reaction comprising: simultaneously performing a plurality of single-molecule, real-time sequencing reactions on a plurality of nucleic acid templates, wherein each of the nucleic acid templates comprises an adaptor region and an insert region, wherein the adaptor region comprises an initiation site and a runway region and the insert region comprises a sequence of interest, wherein the runway region is between the initiation site and the insert region, wherein the runway region comprises at least 200 nucleotides. 2. The method of claim 1 , wherein each initiation site in each of the nucleic acid templates comprises a substantially identical sequence. 3. The method of claim 1 , wherein each runway region in each of the nucleic acid templates comprises a substantially identical sequence. 4. The method of claim 1 , wherein each of the nucleic acid templates comprises a double-stranded region. 5. The method of claim 4 , wherein the double-stranded region comprises the runway region and the insert region. 6. The method of claim 5 , wherein the adaptor region is a hairpin adaptor region. 7. The method of claim 6 , wherein each of the nucleic acid templates comprises hairpins at both ends of the double-stranded region to form a topologically circular molecule. 8. The method of claim 5 , wherein the template is a linear template comprising a single strand overhang on at least one end, wherein the single strand overhang comprises the initiation site. 9. The method of claim 1 , wherein the single-molecule, real-time sequencing reaction comprises nanopore sequencing. 10. The method of claim 1 , wherein the length of the runway region is 500 nucleotides or greater. 11. The method of claim 1 , wherein the length of the runway region is between 200 and 2,000 nucleotides. 12. The method of claim 1 , wherein the runway region comprises at least one modified base that is absent from the insert region. 13. The method of claim 1 , further comprising a barcode region between the runway region and the insert region.
Assignees
Inventors
Classifications
Hairpin oligonucleotides · CPC title
- C12Q1/6874Primary
involving nucleic acid arrays, e.g. sequencing by hybridisation · CPC title
- C12Q1/6869Primary
Methods for sequencing · CPC title
Time · CPC title
specific length of the oligonucleotides · CPC title
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Frequently asked questions
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- What does patent US10081836B2 cover?
- Methods, compositions, and systems are provided that allow for reliable sequencing of the initial sequence region of a sequence of interest. The methods of the invention allow for more reliable barcoding of subpopulations of nucleic acids to be sequenced.
- Who is the assignee on this patent?
- Pacific Biosciences California Inc
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/6874. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Sep 25 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 3 related publications on this page (citations in our corpus or others sharing the same primary CPC).