Devices and methods for contactless dielectrophoresis for cell or particle manipulation

Patent metadata

Field	Value
Publication number	US-10078066-B2
Application number	US-201514636646-A
Country	US
Kind code	B2
Filing date	Mar 3, 2015
Priority date	Mar 9, 2009
Publication date	Sep 18, 2018
Grant date	Sep 18, 2018

Abstract

Devices and methods for performing dielectrophoresis are described. The devices contain sample channel which is separated by physical barriers from electrode channels which receive electrodes. The devices and methods may be used for the separation and analysis of particles in solution, including the separation and isolation of cells of a specific type. As the electrodes do not make contact with the sample, electrode fouling is avoided and sample integrity is better maintained.

First claim

What is claimed is:

1. A dielectrophoresis device comprising:
a channel for receiving a sample fluid;
a first electrode channel configured to receive a first conductive solution and a first electrode;
a first electrode located in the first electrode channel;
a first electrical insulation barrier, which exhibits capacitive behavior between the first electrode channel and the channel for receiving the sample fluid;
a second electrode channel configured to receive a second conductive solution and a second electrode;
a second electrode inserted into the second electrode channel; and
a second electrical insulation barrier, which exhibits capacitive behavior between the second electrode channel and the channel for receiving the sample fluid, and
wherein application of a high-frequency alternating current to the first and second electrodes causes their capacitive coupling to the channel for receiving the sample fluid and an electric field is induced across the sample fluid.

2. The dielectrophoresis device of claim 1 , wherein the channel for receiving the sample fluid, the first electrode channel and the second electrode channel are all formed in the same layer of the device.

3. The dielectrophoresis device of claim 1 , wherein the channel for receiving a sample fluid is linear.

4. The dielectrophoresis device of claim 1 , wherein the channel for receiving a sample fluid is branched.

5. The dielectrophoresis device of claim 1 , wherein the channel for receiving a sample fluid is T-shaped.

6. The dielectrophoresis device of claim 1 , wherein the channel for receiving a sample fluid, the first electrode channel and the second electrode channel are all formed in a single substrate layer, and wherein the first electrical insulation barrier and second electrical insulation barrier are formed by the substrate.

7. The dielectrophoresis device of claim 1 , wherein the first and second electrode channels are filled with a phosphate buffer saline.

8. The dielectrophoresis device of claim 6 , wherein the substrate layer is made from polydimethylsiloxane, glass, polyimide, polycarbonate, silicon or plastic.

9. The dielectrophoresis device of claim 1 , wherein an electrode channel or the channel for receiving a sample fluid comprises an insulation structure.

10. The dielectrophoresis device of claim 1 , wherein
the first electrode channel is in a first substrate layer;
the channel for receiving a sample fluid is in a second substrate layer;
the second electrode channel is in a third substrate layer;
the first electrical insulation barrier is between the first substrate layer and the second substrate layer;
the second electrical insulation barrier is between the second substrate layer and the third substrate layer.

11. The dielectrophoresis device of claim 10 , wherein the first and second substrate layers are made from polydimethylsiloxane, glass, polyimide, polycarbonate, silicon or plastic.

12. The dielectrophoresis device of claim 10 , wherein the insulation barrier is made from polydimethylsiloxane, glass, polyimide, polycarbonate, silicon or plastic.

13. The dielectrophoresis device of claim 10 , wherein the channel for receiving a sample fluid is linear.

14. The dielectrophoresis device of claim 10 , wherein the channel for receiving a sample fluid is branched.

15. The dielectrophoresis device of claim 10 , wherein the channel for receiving a sample fluid is T-shaped.

16. The dielectrophoresis device of claim 10 , wherein the first and second electrode channels are filled with a phosphate buffer saline.

17. The dielectrophoresis device of claim 10 , wherein an electrode channel or the channel for receiving a sample fluid comprises an insulation structure.

Assignees

• Davalos Rafael V
• Shafiee Hadi
• Sano Michael Benjamin
• Caldwell John L
• Virginia Tech Intellectual Properties Inc

Inventors

• Davalos Rafael V
• Shafiee Hadi
• Sano Michael Benjamin
• Caldwell John L

Classifications

• B03C5/026

  using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC] · CPC title

• B03C5/022

  Non-uniform field separators · CPC title

• B03C2201/26

  for use in medical or biological applications · CPC title

• B03C5/005 Primary

  Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength · CPC title

• B01D57/02

  by electrophoresis (treatment of water, waste water, sewage or sludge by electrophoresis C02F1/469; electrophoretic production of compounds or non-metals C25B7/00; investigating or analysing materials by using electrophoresis G01N27/26) · CPC title