Micro-organism detection medium comprising at least one alkyl(thio)glycoside

Patent metadata

Field	Value
Publication number	US-10077464-B2
Application number	US-201314655224-A
Country	US
Kind code	B2
Filing date	Dec 24, 2013
Priority date	Dec 28, 2012
Publication date	Sep 18, 2018
Grant date	Sep 18, 2018

Abstract

A microorganism detection medium, said detection being based on showing the presence of a microbial enzyme activity chosen from esterase and/or osidase and/or peptidase and/or sulfatase and/or phosphatase activities of microorganisms, preferably said microbial enzyme activity being an esterase activity, said medium comprising:
at least one chromogenic and/or fluorogenic substrate specific to the enzyme activity sought, preferably specific to an esterase activity, at least one alkyl(thio)glycoside, at least one solvent (S).

First claim

The invention claimed is:

1. A microorganism detection medium comprising:
at least one chromogenic and/or fluorogenic substrate capable of being hydrolyzed by an esterase;
at least one alkylthioglycoside or at least one alkylglycoside; and
at least one solvent,

wherein the detection medium comprises a culture medium suitable for growing a target microorganism, wherein the at least one alkylthioglycoside or at least one alkylglycoside corresponds to the general formula (I):
R—X-(G) n   (I)

wherein:
R represents a linear or branched, saturated or unsaturated, aliphatic radical containing 6 to 12 carbon atoms;
X is —O— or —S—;
G represents a carbohydrate residue; and
n is an integer between 1 and 10.

2. The medium according to claim 1 , wherein the at least one alkylglycoside is an alkylglycoside selected from the group consisting of n-octyl-β-D-glucopyranoside and n-dodecyl-β-D-maltoside.

3. The medium according to claim 1 , wherein the concentration of the at least one alkylthioglycoside or at least one alkylglycoside in the medium is from 0.5 g/L to 8 g/L.

4. The medium according to claim 1 , wherein the solvent is selected from the group consisting of alcohols, polar aprotic solvents, aqueous solvents, and mixtures thereof.

5. The medium according to claim 1 , wherein the chromogenic and/or fluorogenic substrate comprises:
a target part capable of being hydrolyzed by the enzyme; and
a marker part comprising a chromophore and/or fluorophore.

6. The medium according to claim 1 , wherein the chromogenic and/or fluorogenic substrate comprises indoxyl or derivatives thereof.

7. The medium according to claim 6 , further comprising an agent which promotes the oxidative polymerization of the indoxyl or derivatives thereof.

8. The medium according to claim 1 , wherein the concentration of chromogenic and/or fluorogenic substrate is from 1 mg/L to 10 g/L.

9. The medium according to claim 1 , wherein the culture medium is selected from the group consisting of MacConkey media, Hektoen selective media, selective chromogenic media for selectively detecting salmonella , chromogenic Salmonella media, Columbia ANC media, PALCAM media, Sabouraud gentamycin-chloramphenicol type media, non-selective Columbia +/−blood media, Trypticase Soy Agar (TSA) media, nutrient agar media, and Sabouraud media.

10. The medium according to claim 1 , wherein the at least one alkylglycoside is n-dodecyl-β-D-maltoside.

11. The medium according to claim 1 , wherein n is an integer between 1 and 3.

12. The medium according to claim 7 , wherein the agent comprises an ammonium ferric citrate complex.

13. The medium according to claim 1 , wherein the at least one alkylthioglycoside is n-octyl-β-D-thioglucopyranoside.

14. The medium according to claim 1 , wherein R represents a linear or branched, saturated or unsaturated, aliphatic radical containing 8 to 12 carbon atoms.

Assignees

• Biomerieux Sa
• Biomerieux Sa

Inventors

• Cellier Marie
• Orenga Sylvain
• Perry John

Classifications

• C12Q1/44 Primary

  involving esterase · CPC title

• C12Q1/045 Primary

  Culture media therefor · CPC title

• G01N2333/255

  Salmonella (G) · CPC title

• C12Q1/10

  Enterobacteria · CPC title