Method for characterizing at least one microorganism by means of mass spectrometry

Patent metadata

Field	Value
Publication number	US-10077461-B2
Application number	US-201013502020-A
Country	US
Kind code	B2
Filing date	Oct 14, 2010
Priority date	Oct 15, 2009
Publication date	Sep 18, 2018
Grant date	Sep 18, 2018

Abstract

A method for characterizing at least one microorganism from a sample includes identifying the at least one microorganism and determining the properties of typing, potential resistance to at least one antimicrobial, and virulence factor. The properties of typing, resistance to at least one antimicrobial, and virulence factor for the at least one microorganism are determined by implementing mass spectrometry using proteins, peptides and/or metabolites as markers of the properties of typing, resistance to at least one antimicrobial, and virulence factor.

First claim

The invention claimed is:

1. A method for characterizing a microorganism, comprising:
identifying the microorganism; and
subjecting the microorganism identified to mass spectrometry with a spectrometer in MS/MS mode using multiple reaction monitoring (MRM) to identify markers of properties of typing, potential resistance to at least one antimicrobial, and virulence factor, of the microorganism,
wherein:
the markers are selected from the group consisting of proteins, peptides, and metabolites, and
the microorganism is a bacterium, a virus, a protozoan, or a yeast.

2. The method for characterizing a microorganism of claim 1 , wherein the microorganism is Staphylococcus aureus.

3. The method for characterizing a microorganism of claim 2 , wherein the markers identified comprise at least one peptide that has an amino acid sequence selected from the group consisting of SEQ ID NOs: 2, 13, 15, 21, and 24.

4. The method for characterizing a microorganism of claim 2 , wherein the markers identified comprise at least one peptide that has an amino acid sequence selected from the group consisting of SEQ ID NOs: 13 and 15.

5. The method for characterizing a microorganism of claim 2 , wherein the markers identified comprise at least one peptide that has an amino acid sequence selected from the group consisting of SEQ ID NOs: 21 and 24.

6. The method for characterizing a microorganism of claim 1 , wherein the microorganism is Escherichia coli.

7. The method for characterizing a microorganism of claim 6 , wherein the markers identified comprise at least one peptide that has an amino acid sequence selected from the group consisting of SEQ ID NOs: 64, 66, and 85 to 87.

8. The method for characterizing a microorganism of claim 6 , wherein the markers identified comprise at least one peptide that has an amino acid sequence selected from the group consisting of SEQ ID NOs: 64 and 66.

9. The method for characterizing a microorganism of claim 6 , wherein the markers identified comprise at least one peptide that has an amino acid sequence selected from the group consisting of SEQ ID NOs: 85, 86, and 87.

10. The method for characterizing a microorganism of claim 1 , wherein the microorganism is Candida albicans.

11. The method for characterizing a microorganism of claim 1 , wherein the markers are identified simultaneously.

12. The method for characterizing a microorganism of claim 1 , wherein the markers are identified in the same mass spectrometry apparatus.

13. The method for characterizing a microorganism of claim 1 , wherein the microorganism is identified by mass spectrometry.

14. The method for characterizing a microorganism of claim 13 , wherein the mass spectrometry for identifying the microorganism is selected from the group consisting of MS type, MS/MS type, and MS type followed by MS/MS type.

15. The method for characterizing a microorganism of claim 1 , further comprising:
confirming, by mass spectrometry, the identification of the microorganism.

16. The method for characterizing a microorganism of claim 15 , wherein the mass spectrometry for confirming the identification of the microorganism is MS/MS type.

17. The method for characterizing a microorganism of claim 16 , wherein the mass spectrometry for confirming the identification of the microorganism is MRM type.

18. The method for characterizing a microorganism of claim 1 , wherein:
the markers are peptides, and
the microorganism is a bacterium or a yeast.

19. A method for characterizing a microorganism, comprising:
identifying the microorganism; and
subjecting the microorganism identified to mass spectrometry with a spectrometer in MS/MS mode using multiple reaction monitoring (MRM) to identify at least one marker of a property of typing, potential resistance to at least one antimicrobial, or virulence factor, of the microorganism,
wherein:
the marker is a protein or a peptide, and
the microorganism is a bacterium, a virus, a protozoan, or a yeast.

20. A method for characterizing a microorganism, comprising:
identifying the microorganism; and
subjecting the microorganism identified to mass spectrometry with a spectrometer in MS/MS mode using multiple reaction monitoring (MRM) to identify at least one marker of a property of typing, potential resistance to at least one antimicrobial, or virulence factor, of the microorganism,
wherein:
the marker is selected from the group consisting of proteins, peptides, and metabolites, and
the microorganism is a virus, a protozoan, or a yeast.

Assignees

• Beaulieu Corinne
• Charretier Yannick
• Charrier Jean Philippe
• Chatellier Sonia
• Dufour Philippe
• Franceschi Christine
• Girard Victoria
• Pons Sylvie
• Biomerieux Sa

Inventors

• Beaulieu Corinne
• Charretier Yannick
• Charrier Jean-Philippe
• Chatellier Sonia
• Dufour Philippe
• Franceschi Christine
• Girard Victoria
• Pons Sylvie

Classifications

• C12Q1/14

  Streptococcus; Staphylococcus · CPC title

• G01N2333/245

  Escherichia (G) · CPC title

• G01N33/6851

  Methods of protein analysis involving laser desorption ionisation mass spectrometry · CPC title

• C12Q1/04 Primary

  Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor {(C12Q1/6897 takes precedence)} · CPC title

• G01N2333/40

  from Candida · CPC title