Enzymatic systems for carbon fixation and methods of generating same

Patent metadata

Field	Value
Publication number	US-10077437-B2
Application number	US-201615189021-A
Country	US
Kind code	B2
Filing date	Jun 22, 2016
Priority date	Feb 11, 2010
Publication date	Sep 18, 2018
Grant date	Sep 18, 2018

Abstract

A system for carbon fixation is provided. The system comprises enzymes which catalyze reactions of a carbon fixation pathway, wherein at least one of the reactions of the carbon fixation pathway is a carboxylation reaction, wherein products of the reactions of the carbon fixation pathway comprise oxaloacetate and malonyl-CoA, wherein an enzyme which performs the carboxylation reaction is selected from the group consisting of phophoenolpyruvate (PEP) carboxlase, pyruvate carboxylase and acetyl-CoA carboxylase and wherein an export product of the carbon fixation pathway is glyoxylate. Additional carbon fixation pathways are also provided and methods of generating same.

First claim

What is claimed is:

1. A system for carbon fixation, comprising an electron donor and enzymes which catalyze reactions of a carbon fixation pathway, wherein all the carboxylation reactions of the carbon fixation pathway utilize:
(i) phophoenolpyruvate (PEP) carboxylase and acetyl-CoA carboxylase; or
(ii) phophoenolpyruvate (PEP) carboxylase; or
(iii) pyruvate carboxylase and acetyl-CoA carboxylase; or
(iv) pyruvate carboxylase
wherein products of said reactions of the carbon fixation pathway comprise oxaloacetate and malonyl-CoA, and wherein an additional product of the carbon fixation pathway is glyoxylate, wherein an in-organic carbon is introduced into a substrate to become a carboxylic acid group during said carboylation reactions, wherein said system for carbon fixation is in a non-cellular particle.

2. The system of claim 1 , wherein said glyoxylate is an export product.

3. The system of claim 1 , wherein an export product of the carbon fixation pathway is pyruvate.

4. The system of claim 1 , wherein the enzymes of the carbon fixation pathway generate more than 0.3 μmol glyceraldehyde-3-phosphate/min/mg.

5. The system of claim 1 , wherein said enzyme which performs said carboxylation enzyme is PEP carboxylase.

6. The system of claim 1 , wherein at least two of said reactions of the carbon fixation pathway are carboxylation reactions.

7. The system of claim 1 , wherein one of said reactions of the carbon fixation pathway utilizes methylmalonyl-CoA carboxytransferase.

8. The system of claim 1 , wherein products of the reactions of the carbon fixation pathway further comprise pyruvate, phophoenolpyruvate (PEP), malate, malyl CoA and acetyl CoA.

9. The system of claim 1 , wherein said electron donor is selected from the group consisting of ATP, NADH and NADPH.

10. The system of claim 1 wherein said non-cellular particle is selected from the group consisting of polymeric particles, microcapsules liposomes, microspheres, microemulsions, nano-plates, nanoparticles, nanocapsules and nanospheres.

11. The system of claim 1 , wherein the enzymes of said carboxylation reactions of the carbon fixation pathway consist of:
(i) PEP carboxylase and acetyl-CoA carboxylase; or
(ii) PEP carboxylase; or
(iii) pyruvate carboxylase and acetyl-CoA carboxylase; or
(iv) pyruvate carboxylase.

Assignees

• Yeda Res & Dev

Inventors

• Milo Ron
• Bar-Even Arren
• Noor Elad

Classifications

• C12N15/70

  Vectors or expression systems specially adapted for E. coli · CPC title

• C12Y201/03001

  Methylmalonyl-CoA carboxytransferase (2.1.3.1) · CPC title

• C12Y604/01001

  Pyruvate carboxylase (6.4.1.1) · CPC title

• C12Y401/01031

  Phosphoenolpyruvate carboxylase (4.1.1.31) · CPC title

• C12N9/1205

  Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases · CPC title