Paramagnetic supports for use as assay reagents

Patent metadata

Field	Value
Publication number	US-10077376-B2
Application number	US-201515113920-A
Country	US
Kind code	B2
Filing date	Jan 29, 2015
Priority date	Jan 31, 2014
Publication date	Sep 18, 2018
Grant date	Sep 18, 2018

Abstract

A composition for use as an assay reagent includes a paramagnetic solid support comprising a coating of a synthetic copolymer. The synthetic copolymer comprises two or three of a first copolymerized monomer, a second copolymerized monomer and a third copolymerized monomer and further comprises a polyethylenic backbone.

First claim

What is claimed is:

1. A composition for use as an assay reagent, the composition comprising:
a paramagnetic metal oxide solid support comprising a coating of a synthetic copolymer wherein the synthetic copolymer comprises three of a first copolymerized monomer, a second copolymerized monomer and a third copolymerized monomer and wherein the synthetic copolymer comprises a polyethylenic backbone and wherein:
(i) the first copolymerized monomer comprises a pendant moiety of the formula: —C(O)—O—(CH 2 CH 2 O) m —Z, wherein:
Z is H or alkyl of from 1 to 6 carbon atoms,
m is 1 to 100;
(ii) the second copolymerized monomer comprises a pendant moiety of the formula:
—OC(O)NR-J wherein R is H or alkyl of from 1 to 6 carbon atoms and J is a member of a specific binding pair; and
(iii) the third copolymerized monomer comprises a pendant moiety of the formula: —COOH or a derivative thereof.

2. The composition according to claim 1 wherein the paramagnetic solid support is a particle.

3. The composition according to claim 2 wherein the metal oxide metal of the metal oxide particle is a paramagnetic metal selected from the group consisting of iron, chromium, lithium, sodium, magnesium, aluminum, manganese, strontium, zirconium, molybdenum, ruthenium, rhodium, palladium, tin, samarium, europium, tungsten, and platinum.

4. The composition according to claim 1 wherein the polyethylenic backbone has the formula —CH 2 —CHR 9 − wherein R 9 is H or alkyl of from 1 to 6 carbon atoms.

5. The composition according to claim 1 wherein a member of a specific binding pair is associated with the solid support.

6. The composition according to claim 5 wherein the member of the specific binding pair is an antibody, an antigen, a hapten, or a small molecule.

Assignees

• Siemens Healthcare Diagnostics Inc

Inventors

• Lele Bhalchandra S
• Allgaier Eric
• Pervere Robert
• Lyons Jeremy
• St-Onge Steve

Classifications

• C09D133/24

  Homopolymers or copolymers of amides or imides · CPC title

• C09D123/0869

  Acids or derivatives thereof · CPC title

• G01N33/78

  Thyroid gland hormones {, e.g. T3, T4, TBH, TBG or their receptors} · CPC title

• C08F230/02

  containing phosphorus · CPC title

• C08F2/06

  Organic solvent · CPC title