Bubble removal method and bubble removal device

Patent metadata

Field	Value
Publication number	US-10077124-B2
Application number	US-201514667179-A
Country	US
Kind code	B2
Filing date	Mar 24, 2015
Priority date	Sep 24, 2012
Publication date	Sep 18, 2018
Grant date	Sep 18, 2018

Abstract

A method for removing bubbles from an observation area includes pressing, with a pressing member, a part of an upper surface of the container that is positioned above the observation area, and moving the pressing member so as to push the bubbles outside the observation area.

First claim

The invention claimed is:

1. A method for removing bubbles from an observation area that is a part of space in a container, the method comprising:
pressing a pressing member horizontally placed on a part of an upper surface of the container that is positioned above the observation area,
wherein the pressing member is a plate-like member, and
wherein the container is made of a soft package material and encloses a liquid comprising the bubbles;

providing a reciprocal movement of the pressing member one or more times so as to push the bubbles outside the observation area,
wherein each reciprocal movement comprises moving the pressing member from an upper side of the container to a lower side of the container, moving the pressing member from the lower side to the upper side, and moving the pressing member from the upper side to the lower side; and

stopping the pressing member at a position where the pressing member is moved at the lower side.

2. The method for removing bubbles according to claim 1 , the method further comprising, prior to providing the reciprocal movement:
pressing the pressing member at an angle against the part of the upper surface of the container that is positioned above the observation area; and
moving the pressing member until the pressing member becomes horizontal relative to the upper surface of the container.

3. The method for removing bubbles according to claim 1 , the method further comprising, prior to providing the reciprocal movement:
pressing the pressing member at an angle against the part of the upper surface of the container that is positioned above the observation area; and
swinging the pressing member between a position where the pressing member is held at the angle and a position where the pressing member is horizontal relative to the upper surface of the container.

4. The method for removing bubbles according to claim 1 , the method further comprising:
pressing a rod-like member horizontally against the upper surface of the container, and
moving the rod-like member in the horizontal direction so as to pass the pressing members over the part of the upper surface of the container that is positioned above the observation area.

5. An apparatus for removing bubbles from an observation area that is a part of space in a container, the apparatus comprising:
a mounting table comprising an observation range formed of a transparent member or a hole for observing a part of space in the container that is positioned above the observation range as an observation area when the container is put on the mounting table;
a pressing member that presses a part of an upper surface of the container that is positioned above the observation area, wherein the pressing member is a plate-like member;
and a driving unit that moves the pressing member so as to push the bubbles outside the observation area, wherein the driving unit is configured to perform:
pressing the pressing member horizontally placed on the part of the upper surface of the container that is positioned above the observation area;
providing a reciprocal movement of the pressing member one or more times so as to push the bubbles outside the observation area, wherein each reciprocal movement comprises moving the pressing member from an upper side of the container to a lower side of the container, moving the pressing member from the lower side to the upper side, and moving the pressing member from the upper side to the lower side: and stopping the pressing member at a position where the pressing member is moved at the lower side.

6. The apparatus for removing bubbles according to claim 5 , wherein prior to providing the reciprocal movement,
the driving unit presses the pressing member at an angle against the part of the upper surface of the container that is positioned above the observation area and moves the pressing member until the pressing member becomes horizontal relative to the upper surface of the container.

7. The apparatus for removing bubbles according to claim 5 , wherein prior to providing the reciprocal movement,
the driving unit presses the pressing member at an angle against the part of the upper surface of the container that is positioned above the observation area and swings the pressing member between a position where the pressing member is held at the angle and a position where the pressing member is horizontal relative to the upper surface of the container.

8. The apparatus for removing bubbles, according to claim 5 , further comprising another pressing member and another driving unit wherein
the another pressing members is a rod-like member, and
the another driving unit presses horizontally against the upper surface of the container and moves the rod-like member in the horizontal direction so as to pass the rod-like member over the part of the upper surface of the container that is positioned above the observation area.

9. The method for removing bubbles according to claim 1 , wherein the container is a culture bag containing a culture liquid.

10. The apparatus for removing bubbles according to claim 5 , wherein the container is a culture bag containing a culture liquid.

Assignees

• Toyo Seikan Group Holdings Ltd

Inventors

• Suenaga Ryo
• Tanaka Satoshi
• Ota Kyohei

Classifications

• B01L3/505 Primary

  Flexible containers without fluid transport within · CPC title

• B65B9/042 Primary

  for fluent material · CPC title

• B01L2400/0481

  squeezing of channels or chambers · CPC title

• C12M41/36

  of biomass, e.g. colony counters or by turbidity measurements (electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404) · CPC title

• B01L2300/0654

  Lenses; Optical fibres · CPC title