Apparatus and method for cell kill confirmation
US-12168779-B2 · Dec 17, 2024 · US
US10074957B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10074957-B2 |
| Application number | US-201715431111-A |
| Country | US |
| Kind code | B2 |
| Filing date | Feb 13, 2017 |
| Priority date | Jan 19, 2008 |
| Publication date | Sep 11, 2018 |
| Grant date | Sep 11, 2018 |
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A method of providing optical supercontinuum pulses can comprise generating optical pump pulses with an optical pump laser, the optical pump pulses having a pump pulse repetition rate; launching optical pump pulses into a nonlinear optical element comprising an optical fiber; generating optical supercontinuum pulses from the optical pump pules via spectral broadening within the optical fiber; selectively providing a plurality of different repetition rates for the optical pump pulses so as to generate optical supercontinuum pulses having different repetition rates; and providing nominally identical spectral broadening of the optical supercontinuum pules having the different repetition rates.
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The invention claimed is: 1. A method of providing optical supercontinuum pulses, comprising: generating optical pump pulses with an optical pump source; launching optical pump pulses into a nonlinear optical element comprising an optical fiber; generating optical supercontinuum pulses from the optical pump pulses via spectral broadening within the optical fiber; selectively providing a plurality of different repetition rates for the optical pump pulses so as to generate optical supercontinuum pulses having different repetition rates; and providing nominally identical spectral broadening of the optical pump pulses having the different repetition rates. 2. The method of providing optical supercontinuum pulses of claim 1 , comprising using the optical supercontinuum pulses to provide illumination in an application to excite fluorescence. 3. The method of providing optical supercontinuum pulses of claim 2 , comprising measuring a decay lifetime of the excited fluorescence. 4. The method of providing optical supercontinuum pulses of claim 2 , wherein the application comprises fluorescence lifetime imaging microscopy (FLIM). 5. The method of providing optical supercontinuum pulses of claim 2 , wherein the application comprises stimulated emission depletion (STED) microscopy. 6. The method of providing optical supercontinuum pulses of claim 1 , comprising wavelength filtering the optical supercontinuum pulses at a predetermined wavelength. 7. The method of providing optical supercontinuum pulses of claim 1 , comprising launching the optical pump pulses into the optical fiber without the use of free space optics. 8. The method of providing optical supercontinuum pulses of claim 1 , comprising amplifying the optical pump pulses prior to launching the optical pump pulses into the nonlinear optical element. 9. The method of providing optical supercontinuum pulses of claim 8 , wherein the amplified optical pump pulses have the plurality of different repetition rates prior to launching the amplified optical pump pulses into the nonlinear optical element. 10. A method of providing optical supercontinuum pulses, comprising: generating optical pump pulses with an optical pump source, an optical pump pulse having a pulse energy; launching optical pump pulses into a nonlinear optical element comprising an optical fiber; generating optical supercontinuum pulses from the optical pump pulses via spectral broadening within the optical fiber; selectively providing a plurality of different repetition rates for the optical pump pulses so as to generate optical supercontinuum pulses having different repetition rates; and wherein the pulse energy is fixed as the pulse to pulse separation is increased with reducing repetition rate. 11. The method of providing optical supercontinuum pulses of claim 10 , comprising using the optical supercontinuum pulses to provide illumination in an application to excite fluorescence. 12. The method of providing optical supercontinuum pulses of claim 11 , comprising measuring a decay lifetime of the excited fluorescence. 13. The method of providing optical supercontinuum pulses of claim 11 , wherein the application is fluorescence lifetime imaging microscopy (FLIM). 14. The method of providing optical supercontinuum pulses of claim 11 , wherein the application is stimulated emission depletion (STED) microscopy. 15. The method of providing optical supercontinuum pulses of claim 10 , comprising wavelength filtering the optical supercontinuum pulses at a predetermined wavelength. 16. The method of providing optical supercontinuum pulses of claim 10 , comprising launching the optical pump pulses into the optical fiber without the use of free space optics. 17. The method of providing optical supercontinuum pulses of claim 10 , comprising amplifying the optical pump pulses prior to launching the pump pulses into the nonlinear optical element. 18. A supercontinuum pulse source, comprising: an optical pump source for generating optical pump pulses; the optical pump pulses having a selectively variable pump pulse repetition rate so that optical pump pulses can have different repetition rates; a nonlinear optical element comprising an optical fiber arranged to receive the optical pump pulses and spectrally broaden the optical pump pulses to generate optical supercontinuum pulses; wherein the optical pump pulses having different repetition rates generate optical supercontinuum pulses having different repetition rates; and wherein optical pump pulses having different repetition rates are nominally identically spectrally broadened by the nonlinear optical element. 19. A supercontinuum pulse source, comprising: an optical pump source operable to generate optical pump pulses, an optical pump pulse having a pump pulse energy; a nonlinear optical element comprising an optical fiber arranged to receive the optical pump pulses and spectrally broaden the optical pump pulses to generate optical supercontinuum pulses; the optical pump pulses further comprising a plurality of different repetition rates, wherein the optical pump pulses having different repetition rates generate optical supercontinuum pulses having different repetition rates; wherein the optical pulse energy is fixed as the pulse to pulse separation is increased with reducing repetition rate.
Fluorescence microscopy (fluorescence microscopes per se G02B21/0076 and G02B21/16) · CPC title
Excitation at two or more wavelengths · CPC title
Amplifier arrangements, e.g. MOPA · CPC title
Pulsed lasers · CPC title
Pulse repetition rate control (H01S3/11 takes precedence) · CPC title
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