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US-2024158744-A1 · May 16, 2024 · US
Method for generation of a cell composition of mesencephalic dopaminergic progenitor cells
US10072245B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10072245-B2 |
| Application number | US-201615055414-A |
| Country | US |
| Kind code | B2 |
| Filing date | Feb 26, 2016 |
| Priority date | Feb 27, 2015 |
| Publication date | Sep 11, 2018 |
| Grant date | Sep 11, 2018 |
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Abstract
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The present invention provides a method for generation of a cell composition of mesencephalic dopaminergic progenitor cells from a starting cell composition comprising pluripotent and/or multipotent stem cells, the method comprising the steps of a) differentiating said pluripotent and/or multipotent stem cells into mesencephalic dopaminergic progenitor cells, thereby generating a cell population comprising mesencephalic dopaminergic progenitor cells and other cells, b) dissociating the differentiated cells of step a) into a single cell suspension, and c) enriching said mesencephalic dopaminergic progenitor cells by using an antigen binding molecule specific for the CD47 antigen for positive selection of said mesencephalic dopaminergic progenitor cells in said single cell suspension. Said method may be performed in a closed cell sample processing system and may be performed in an automated manner.
First claim
Opening claim text (preview).
What is claimed is: 1. A method for generating a cell composition of mesencephalic dopaminergic progenitor cells from a starting cell composition comprising pluripotent and/or multipotent stem cells, the method comprising the steps of a) differentiating said pluripotent and/or multipotent stem cells into mesencephalic dopaminergic progenitor cells, thereby generating a cell population comprising mesencephalic dopaminergic progenitor cells and other cells, b) dissociating the differentiated cells of step a) into a single cell suspension, c) enriching said mesencephalic dopaminergic progenitor cells by using an antigen binding molecule specific for the CD47 antigen for positive selection of said mesencephalic dopaminergic progenitor cells in said single cell suspension. 2. The method according to claim 1 , wherein said pluripotent and/or multipotent stem cells are proliferated in a proliferation step before step a). 3. The method according to claim 1 , wherein said method is performed in a closed cell sample processing system. 4. The method according to claim 3 , wherein said method is an automated method. 5. The method according to claim 1 , wherein said antigen binding molecule specific for the CD47 antigen is an anti-CD47 antibody or antigen binding fragment thereof. 6. The method according to claim 1 , wherein the step of enrichment of said mesencephalic dopaminergic progenitor cells is performed by fluorescent activated cell sorting or magnetic cell sorting. 7. The method of claim 1 , wherein the mesencephalic dopaminergic progenitor cells are human or non-human primate cells.
Assignees
Inventors
Classifications
- C12N5/0623Primary
Stem cells · CPC title
from non-embryonic pluripotent stem cells · CPC title
Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue · CPC title
with CD designations not provided for elsewhere · CPC title
- C12N5/0619Primary
Neurons · CPC title
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- What does patent US10072245B2 cover?
- The present invention provides a method for generation of a cell composition of mesencephalic dopaminergic progenitor cells from a starting cell composition comprising pluripotent and/or multipotent stem cells, the method comprising the steps of a) differentiating said pluripotent and/or multipotent stem cells into mesencephalic dopaminergic progenitor cells, thereby generating a cell populatio…
- Who is the assignee on this patent?
- Miltenyi Biotec Gmbh
- What technology area does this patent fall under?
- Primary CPC classification C12N5/0623. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Sep 11 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).