Derivation of embryonic stem cells and embryo-derived cells

Patent metadata

Field	Value
Publication number	US-10072243-B2
Application number	US-201715448571-A
Country	US
Kind code	B2
Filing date	Mar 2, 2017
Priority date	Nov 4, 2004
Publication date	Sep 11, 2018
Grant date	Sep 11, 2018

Abstract

This present invention provides novel methods for deriving embryonic stem cells and embryo-derived cells from an embryo without requiring destruction of the embryo. The invention further provides cells and cell lines derived without embryo destruction, and the use of the cells for therapeutic and research purposes. It also relates to novel methods of establishing and storing an autologous stem cell line prior to implantation of an embryo, e.g., in conjunction with reproductive therapies such as IVF.

First claim

We claim:

1. A method of producing human embryonic stem (ES) cells, comprising:
(a) culturing a blastomere obtained from a human embryo in medium containing less than 5 mM glucose to generate a cluster of two or more blastomeres;
(b) directly or indirectly contacting the cultured cluster of two or more blastomeres with embryonic or fetal cells; and
(c) culturing the cluster of two or more blastomeres of (b) to produce ES cells.

2. A method of producing human embryonic stem (ES) cells, comprising:
(a) culturing a blastomere obtained from a human embryo in medium containing less than 5 mM glucose to generate a cluster of two or more blastomeres;
(b) directly or indirectly contacting the cultured cluster of two or more blastomeres with medium sufficient to promote the growth and survival of the cultured cluster of two or more blastomeres that is supplemented with ACTH; and
(c) culturing the cluster of two or more blastomeres of (b) to produce ES cells.

3. The method of claim 2 , wherein (a) comprises culturing a blastomere obtained from a human embryo in medium containing less than 5 mM glucose and having a osmolarity of less than 310 mOsm to generate a cluster of two or more blastomeres.

4. The method of claim 2 , wherein the medium sufficient to promote the growth and survival of the cultured cluster of two or more blastomeres is medium conditioned with embryonic or fetal cells.

Assignees

• Astellas Inst For Regenerative Medicine

Inventors

• Chung Young Gie
• Lanza Robert P
• Klimanskaya Irina V

Classifications

• C12N5/0603

  Embryonic cells (production of embryos, nuclear transfer A01K67/027); Embryoid bodies · CPC title

• C12N2502/02

  embryonic cells · CPC title

• A61K35/12

  Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells (vaccines or medicinal preparations containing antigens or antibodies A61K39/00) · CPC title

• C12N5/0604

  Whole embryos; Culture medium therefor · CPC title

• C12N2500/34

  Sugars · CPC title