Methods and materials for the production of monomers for nylon-4/polyester production

Patent metadata

Field	Value
Publication number	US-10072150-B2
Application number	US-201514976966-A
Country	US
Kind code	B2
Filing date	Dec 21, 2015
Priority date	Dec 22, 2014
Publication date	Sep 11, 2018
Grant date	Sep 11, 2018

Abstract

This document describes biochemical pathways for producing 4-hydroxybutyrate, 4-aminobutyrate, putrescine or 1,4-butanediol by forming one or two terminal functional groups, comprised of amine or hydroxyl group, in a C5 backbone substrate such as 2-oxoglutarate or L-glutamate.

First claim

What is claimed is:

1. A non-naturally occurring method of producing 1,4-butanediol, said method comprising
[1] enzymatically converting 4-hydroxybutyrate to 4-hydroxybutanal using a carboxylate reductase (EC 1.2.99.6), and
[2] enzymatically converting 4-hydroxybutanal to 1,4-butanediol using an alcohol dehydrogenase (EC 1.1.1.1, EC 1.1.1.2, EC 1.1.1.21, or EC 1.1.1.184).

2. The method of claim 1 , wherein 4-hydroxybutyrate is enzymatically synthesized from L-glutamate or 2-oxoglutarate.

3. The method of claim 2 , wherein:
L-glutamate is enzymatically converted to 4-aminobutyrate;
4-aminobutyrate is enzymatically converted to succinate semialdehyde; and
succinate semialdehyde is enzymatically converted to 4-hydroxybutyrate.

4. The method of claim 3 , wherein L-glutamate is enzymatically converted to 4-hydroxybutyrate using (i) a glutamate decarboxylase; (ii) a ω-transaminase; and (iii) a dehydrogenase selected from a 4-hydroxybutyrate dehydrogenase and a 5-hydroxyvalerate dehydrogenase.

5. The method of claim 2 , wherein:
(a) 2-oxoglutarate is enzymatically converted to succinate semialdehyde and succinate semialdehyde is enzymatically converted to 4-hydroxybutyrate; or
(b) 2-oxoglutarate is enzymatically converted to L-glutamate, L-glutamate is enzymatically converted to 4-aminobutyrate, 4-aminobutyrate is enzymatically converted to succinate semialdehyde, and succinate semialdehyde is enzymatically converted to 4-hydroxybutyrate.

6. The method of claim 5 , wherein:
(a) 2-oxoglutarate is enzymatically converted to succinate semialdehyde using a 2-oxoglutarate decarboxylase; and/or
(b) succinate semialdehyde is enzymatically converted to 4-hydroxybutyrate using a 4-hydroxybutyrate dehydrogenase or a 5-hydroxyvalerate dehydrogenase.

7. The method of claim 3 , wherein L-glutamate is enzymatically converted to 4-aminobutyrate using a glutamate decarboxylase having at least 70% sequence identity to the amino acid sequence set forth in SEQ ID NO: 14 or SEQ ID NO: 19.

8. The method of claim 1 , wherein:
(a) said carboxylate reductase has at least 70% sequence identity to the amino acid sequence set forth in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, or SEQ ID NO: 18; and/or
(b) said alcohol dehydrogenase is classified under EC 1.1.1.1, EC 1.1.1,2, EC 1.1.1.21, or EC 1.1.1.184.

9. The method of claim 4 , wherein said ω-transaminase has at least 70% sequence identity to an amino acid sequence set forth in SEQ ID NO 6, SEQ D NO: 7, SEQ D NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, or SEQ ID NO: 11 or is any other ω-transaminase classified under EC 2.6.1.18, EC 2.6.1.19, EC 2.6.1.29, 2.6.1.48, EC 2.6.1.76, EC 2.6.1.82, or EC 2.6.1.96.

10. The method of claim 1 , wherein said method, in all or in part, is performed in a recombinant host by fermentation.

11. The method of claim 10 , wherein:
(a) said host is subjected to a cultivation strategy under aerobic, anaerobic, or micro-aerobic cultivation conditions;
(b) said host is cultured under conditions of nutrient limitation;
(c) said host is retained using a ceramic membrane;
(d) the principal carbon source fed to the fermentation derives from a biological feedstock; and/or
(e) the principal carbon source fed to the fermentation derives from a non-biological feedstock.

12. The method of claim 11 , wherein:
(a) the biological feedstock is, or derives from, monosaccharides, disaccharides, lignocellulose, hemicellulose, cellulose, lignin, levulinic acid, formic acid, triglycerides, glycerol, fatty acids, agricultural waste, condensed distillers' solubles, or municipal wasteful; or
(b) the non-biological feedstock is, or derives from, natural gas, syngas, CO 2 /H 2 , methanol, ethanol, benzoate, non-volatile residue (NVR) caustic wash waste stream from cyclohexane oxidation processes, or terephthalic acid/isophthalic acid mixture waste streams.

13. The method of claim 10 , wherein the host is a prokaryote or a eukaryote.

14. The method of claim 13 , wherein:
(a) said prokaryote is from a genus selected from Escherichia, Clostridia, Corynebacteria, Cupriavidus, Pseudomonas, Delftia, Bacillus, Lactobacillus, Lactococcus , and Rhodococcus ; or
(b) said eukaryote is from a genus selected from Aspergillus, Saccharomyces, Pichia, Yarrowia, Issatchenkia, Debarvomyces, Ancula, and Kluyveromyces.

15. The method of claim 14 , wherein:
(a) said prokaryote is selected from Escherichia coli, Clostridium ljungdahlii, Clostridium autoethanogenum, Clostridium kluyveri, Corynebacterium glutamicum, Cupriavidus necator, Cupriavidus metallidurans, Pseudomonas fluorescens, Pseudomonas putida, Pseudomonas oleavorans, Delftia acidovorans, Bacillus subtillis, Lactobacillus delbrueckii, Lactococcus lactis , and Rhodococcus equi ; or
(b) said eukaryote is selected from Aspergillus niger, Saccharomyces cerevisiae, Pichia pastoris, Yarrowia lipolytica, Issathenkia orientalis, Debaryomyces hansenii, Arxula adenoinivorans , and Kluyveromyces lactis.

16. The method of claim 10 , wherein:
(a) said host comprises one or more of the following attenuated enzymes: a polyhydroxyalkanoate synthase, a those phosphate isomerase, a glucose-6-phosphate isomerase, a transhydrogenase, an NADH-specific glutamate dehydrogenase, or an NADH/NADPH-utilizing glutamate dehydrogenase; and/or
(b) said host overexpresses one or more genes encoding: a phosphoenolpyruvate carboxylase, a pyruvate carboxylase, a 6-phosphogluconate dehydrogenase, a transketolase, a puridine nucleotide transhydrogenase, a formate dehydrogenase, a glyceraldehyde-3P-dehydrogenase, a malic enzyme, a glucose dehydrogenase, a glucose-6-phosphate dehydrogenase, a fructose 1,6 diphosphatase, a L-alanine dehydrogenase, a L-glutamate dehydrogenase, a L-glutamine synthetase, a lysine transporter, a dicarboxylate transporter, and/or a multidrug transporter.

Assignees

• Invista North America Sarl
• Invista North America Sarl

Inventors

• Conradie Alex Van Eck
• Botes Adriana Leonora

Classifications

• C07C229/12

  to carbon atoms of acyclic carbon skeletons · CPC title

• C12N9/88

  Lyases (4.) · CPC title

• C07C227/18

  by reactions involving amino or carboxyl groups, e.g. hydrolysis of esters or amides, by formation of halides, salts or esters · CPC title

• C08G69/08

  derived from amino-carboxylic acids · CPC title

• C08G69/26

  derived from polyamines and polycarboxylic acids · CPC title