Apparatus and methods for creating a static and traversing thermal gradient on a microfluidic device

Patent metadata

Field	Value
Publication number	US-10071374-B2
Application number	US-201414904135-A
Country	US
Kind code	B2
Filing date	Aug 4, 2014
Priority date	Aug 5, 2013
Publication date	Sep 11, 2018
Grant date	Sep 11, 2018

Abstract

A microfluidic device, for use in separation systems, includes a substrate having a fluidic channel. One or more heaters made of a thick film material are integrated with the substrate and in thermal communication with the fluidic channel of the substrate. The one or more heaters produce a thermal gradient within the fluidic channel in response to a current flowing through the one or more heaters. A plurality of electrically conductive taps can be in electrically conductive contact with the one or more heaters. The plurality of electrically conductive taps provides an electrically conductive path to the one or more heaters by which an electrical supply can produce the current flowing through the one or more heaters. Alternatively, the thick film material can be ferromagnetic, and the electrical supply can use induction to cause the current to flow through the one or more heaters.

First claim

What is claimed is:

1. A microfluidic device for use in separation systems, comprising:
a substrate having a fluidic channel that is a chromatography column; and
a heater made of a thick film material integrated with the substrate and in thermal communication with the chromatography column of the substrate, the chromatography column having a length longer than a width and the heater configured to produce a thermal gradient along the length of the chromatography column in response to a current flowing through the heater, the heater extending lengthwise along a length of the microfluidic device.

2. The microfluidic device of claim 1 , wherein the heater is made of a thick film material formed within or on the substrate.

3. The microfluidic device of claim 1 , wherein the heater is trapezoidal in shape with a narrow end and a wide end, and wherein the trapezoidal shape of the heater produces a warm-to-cool thermal gradient from the narrow end to the wide end.

4. The microfluidic device of claim 1 , wherein the substrate further includes one or more channels formed therein that operate as a thermal break for the thermal gradient produced by the heater.

5. The microfluidic device of claim 1 , wherein the substrate further includes one or more channels formed therein that each operate as a thermal break for the thermal gradient produced by the heater, the one or more thermal breaks producing multiple thermal zones on the microfluidic device, and wherein the fluidic channel of the substrate traverses a portion of each of the multiple thermal zones.

6. The microfluidic device of claim 5 , wherein the fluidic channel of the substrate has a spiral shape in one of the multiple thermal zones that transitions to a serpentine shape in another of the multiple thermal zones.

7. The microfluidic device of claim 5 , wherein a pitch of the fluidic channel of the substrate varies within one or more of the multiple thermal zones.

8. The microfluidic device of claim 1 , wherein the fluidic channel traverses the thermal gradient formed by the heater.

9. The microfluidic device of claim 1 , further comprising a cooling element in thermally conductive contact with a first region of the substrate to cool that first region and to shape the thermal gradient within a second region of the substrate bounded by the cooling element.

10. The microfluidic device of claim 1 , further comprising a temperature sensor made of thick film material integrated with the substrate of the microfluidic device and in thermal communication with the heater.

11. The microfluidic device of claim 1 , further comprising one or more of a resistor, conductor, inductor, or dielectric made of thick film material integrated with the substrate.

12. The microfluidic device of claim 1 , wherein the heater is a first heater and the microfluidic device further comprises a second heater spatially separated from the first heater, the first heater being disposed above the second heater, each heater being independently operable to contribute to a shape of the thermal gradient within the fluidic channel.

13. The microfluidic device of claim 1 , wherein the thick film material is ferromagnetic.

14. The microfluidic device of claim 1 , wherein the chromatography column is positioned in a directionally convergent manner with respect to the heater.

15. The microfluidic device of claim 1 , wherein one lengthwise end of the chromatography column is closer to the heater than another lengthwise end of the chromatography column.

16. The microfluidic device of claim 15 , wherein the one lengthwise end of the chromatography column that is closer to the heater is exposed to a warmer temperature than the another lengthwise end of the chromatography column.

Assignees

• Waters Technologies Corp

Inventors

• Michienzi Joseph D
• Murphy James P
• Fogwill Michael O
• Fadgen Keith
• Gerhardt Geoff
• Bertone Gary W

Classifications

• B01L2200/0652

  Sorting or classification of particles or molecules · CPC title

• B01L2300/1811

  using electromagnetic induction heating · CPC title

• B01L3/502707 Primary

  characterised by the manufacture of the container or its components · CPC title

• B01L2300/1894

  Cooling means; Cryo cooling · CPC title

• B01L2300/1805

  Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks · CPC title