Method of identifying an agent that promotes bone growth or increases bone density

Patent metadata

Field	Value
Publication number	US-10071135-B2
Application number	US-201514849313-A
Country	US
Kind code	B2
Filing date	Sep 9, 2015
Priority date	Nov 23, 2005
Publication date	Sep 11, 2018
Grant date	Sep 11, 2018

Abstract

In certain aspects, the present invention provides compositions and methods for promoting bone growth and increasing bone density.

First claim

We claim:

1. A method of identifying an agent that promotes bone growth or increases bone density, the method comprising: a) identifying an agent that binds to a ligand-binding domain of an ActRIIa polypeptide competitively with an activin polypeptide; and b) evaluating the effect of the agent on bone tissue.

2. The method of claim 1 , wherein the agent is selected from the group consisting of non-peptidyl organic molecules, peptides, polypeptides, peptidomimetics, sugars, hormones, and nucleic acid molecules.

3. The method of claim 1 , wherein the agent is identified by a biochemical method.

4. The method of claim 1 , wherein the agent is identified by a mechanism based assay.

5. The method of claim 1 , wherein the agent is evaluated in a cell-based assay.

6. The method of claim 1 , wherein the agent is evaluated in an in vivo assay.

7. The method of claim 1 , wherein the ActRIIa polypeptide comprises the amino acid sequence of SEQ ID NO: 2.

8. The method of claim 1 , wherein the ActRIIa polypeptide comprises the amino acid sequence of SEQ ID NO: 3.

9. The method of claim 1 , wherein the ActRIIa polypeptide comprises the amino acid sequence of SEQ ID NO: 7.

10. The method of claim 1 , wherein the ActRIIa polypeptide comprises the amino acid sequence of SEQ ID NO: 12.

11. The method of claim 1 , wherein the N-terminus of the ActRIIa polypeptide is ILGRSETQE set forth in amino acid position 1-9 of SEQ ID NO: 7.

12. The method of claim 1 , wherein the ActRIIa polypeptide consists of the amino acid sequence of SEQ ID NO: 7.

Assignees

• Acceleron Pharma Inc

Inventors

• Knopf John
• Seehra Jasbir

Classifications

• A61P35/00

  Antineoplastic agents · CPC title

• A61P35/04

  specific for metastasis · CPC title

• A61P43/00

  Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title

• A61P19/08

  for bone diseases, e.g. rachitism, Paget's disease · CPC title

• A61P19/00

  Drugs for skeletal disorders · CPC title