Compositions and methods for selectively depleting senescent cells

Patent metadata

Field	Value
Publication number	US-10071087-B2
Application number	US-201515328368-A
Country	US
Kind code	B2
Filing date	Jul 22, 2015
Priority date	Jul 22, 2014
Publication date	Sep 11, 2018
Grant date	Sep 11, 2018

Abstract

The present disclosure provides compositions and methods for selectively killing senescent cells, wherein the composition comprises piperlongumine (PL) or derivative thereof. The selective killing of senescent cells may delay aging and/or treat age-related disorders.

First claim

What is claimed is:

1. A compound of Formula (II):

wherein:
X is selected from the group consisting of C(O), C(S), C(NH) and S(O) 2 ;
Y is selected from the group consisting of O, NH and S;
n is 1;
R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , and R 8 are each independently selected from the group consisting of hydrogen, deuterium, halogen, CF 3 , CN, OH, OCH 3 , OR, SR, NRR, NRCOR, NRCONRR, NRCO 2 R, COR, CO 2 R, NOR, NO 2 , CONRR, OC(O)NRR, SO 2 R, SO 2 NRR, NRSO 2 R, NRSO 2 NRR, C(O)C(O)R, and C(O)CH 2 C(O)R, a substituted or unsubstituted C1 to C6 alkyl, a substituted or unsubstituted C1 to C6 alkenyl, a substituted or unsubstituted C1 to C6 alkynyl, and a substituted or unsubstituted aryl;
R is independently selected from the group consisting of hydrogen, substituted C1-C4 aliphatic moiety, aliphatic moiety containing nitrogen, oxygen, or sulfur, or alternately, two R moieties bound to the same nitrogen atom are optionally taken together with the nitrogen atom to form a 3-7 membered saturated or unsaturated ring having 1-2 additional heteroatoms independently selected from the group consisting of nitrogen, oxygen, or sulfur;
A is selected from the group consisting of

wherein:
R 9 and R 10 are independently selected from the group consisting of hydrogen, deuterium, halogen, CF 3 , CN, OH, OCH 3 , OR′, SR′, NR′R′, NR′COR′, NR′CONR′R′, NR′CO 2 R′, COR′, CO 2 R′, NOR′, NO 2 , CONR′R′, OC(O)NR′R′, SO 2 R, SO 2 NR′R′, NR′SO 2 R′, NR′SO 2 NR′R′, C(O)C(O)R′, and C(O)CH 2 C(O)R′, a substituted or unsubstituted C1 to C6 alkyl, a substituted or unsubstituted C1 to C6 alkenyl, a substituted or unsubstituted C1 to C6 alkynyl, and a substituted or unsubstituted aryl;
R′ is independently selected from the group consisting of hydrogen, substituted C1-C4 aliphatic moiety, aliphatic moiety containing nitrogen, oxygen, or sulfur, or alternately, two R′ moieties bound to the same nitrogen atom are optionally taken together with the nitrogen atom to form a 3-7 membered saturated or unsaturated ring having 1-2 additional heteroatoms independently selected from the group consisting of nitrogen, oxygen, or sulfur;
Optionally, R 9 and R 10 are taken together to form a 5-8 membered saturated or unsaturated ring having 0-3 heteroatoms independently selected from the group consisting of nitrogen, oxygen, or sulfur;
B is

wherein:
R 11 , R 12 , R 13 , R 14 , and R 15 are each independently selected from the group consisting of hydrogen, deuterium, halogen, CF 3 , CN, OH, OCH 3 , OR″, SR″, NR″R″, NR″COR″, NR″CONR″R″, NRCO 2 R″, COR″, CO 2 R″, NOR″, NO 2 , CONR″R″, OC(O)NR″R″, SO 2 R″, SO 2 NR″R″, NR″SO 2 R″, NR″SO 2 NR″R″, C(O)C(O)R″, and C(O)CH 2 C(O)R″, a substituted or unsubstituted C1 to C6 alkyl, a substituted or unsubstituted C1 to C6 alkenyl, a substituted or unsubstituted C1 to C6 alkynyl, and a substituted or unsubstituted aryl;
R″ is independently selected from the group consisting of hydrogen, substituted C1-C4 aliphatic moiety, aliphatic moiety containing nitrogen, oxygen, or sulfur, or alternately, two R″ moieties bound to the same nitrogen atom are optionally taken together with the nitrogen atom to form a 3-7 membered saturated or unsaturated ring having 1-2 additional heteroatoms independently selected from the group consisting of nitrogen, oxygen, or sulfur;
Optionally, R 11 and R 12 , R 12 and R 13 , R 13 and R 14 , and R 14 and R 15 are taken together to form a 4-8 membered saturated or unsaturated ring having 0-3 heteroatoms independently selected from the group consisting of nitrogen, oxygen, or sulfur;
m is an integer from 0-6; and
or B is one or more monocyclic aryl, a 3-7 membered saturated or partially unsaturated carbocyclic ring, an 8-10 membered bicyclic saturated, partially unsaturated or aryl ring, a 5-6 membered monocyclic heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, a 4-7 membered saturated or partially unsaturated heterocyclic ring having 1-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, a 7-10 membered bicyclic saturated or partially unsaturated heterocyclic ring having 1-5 heteroatoms independently selected from the group consisting of nitrogen, oxygen or sulfur, or an 8-10 membered bicyclic heteroaryl ring having 1-5 heteroatoms independently selected from the group consisting of nitrogen, oxygen, or sulfur;
wherein any and all of the substituted described are substituted with one or more groups that are independently a methyl group or methoxy group.

2. The compound of claim 1 , wherein X is selected from the group consisting of C(O) and S(O) 2 .

3. The compound of claim 1 , wherein Y is O.

4. The compound of claim 1 , wherein R 1 is hydrogen.

5. The compound of claim 1 , wherein n is an integer from 0-2.

6. The compound of claim 1 , wherein X is selected from the group consisting of C(O) and S(O) 2 ; Y is O; R 1 is hydrogen and n is an integer from 0-2.

7. The compound of claim 1 , wherein A is selected from the group consisting of

8. The compound of claim 1 , wherein R 9 and R 10 are hydrogen.

9. The compound of claim 1 , wherein R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 8 are hydrogen.

10. The compound of claim 1 , wherein R 11 and R 15 are hydrogen and R 12 , R 13 and R 14 are OR.

Assignees

• Bioventures Llc

Inventors

• Zheng Guangrong
• Zhou Daohong
• Liu Xingui
• Wang Yingying
• Chang Jianhui
• Feng Wei
• Shao Lijian
• Luo Yi

Classifications

• A61K31/55

  having seven-membered rings, e.g. azelastine, pentylenetetrazole · CPC title

• A23L33/10

  using additives (addition of substantially indigestible substances A23L33/21) · CPC title

• C07D401/06

  linked by a carbon chain containing only aliphatic carbon atoms · CPC title

• C07D211/76

  attached in position 2 or 6 · CPC title

• A61K31/4412

  having oxo groups directly attached to the heterocyclic ring · CPC title