Soybean resistant to cyst nematodes

Patent metadata

Field	Value
Publication number	US-10070614-B2
Application number	US-201314404559-A
Country	US
Kind code	B2
Filing date	May 30, 2013
Priority date	May 30, 2012
Publication date	Sep 11, 2018
Grant date	Sep 11, 2018

Abstract

A transgenic soybean resistant to soybean cyst nematode (SCN), or parts thereof, including an artificial DNA construct encoding a serine hydroxymethyltransferase protein (e.g., GmSHMT). Also provided are GmSHMT alleles containing mutations R130P and Y358N along with research and breeding methods and compositions including such.

First claim

What is claimed is:

1. A plant of an agronomically elite soybean variety with soybean cyst nematode (SCN) resistance, comprising an GmSHMT allele containing mutations P130R and N358Y operably linked to a promoter, wherein the promoter comprises a sequence comprises the entire length of SEQ ID NO: 4 having the nucleic acid substitutions C1080T and C2244T and the nucleic acid deletion −2069A, or a functional fragment thereof, wherein the functional fragment comprises the nucleic acid substitutions C1080T and C2244T and the nucleic acid deletion −2069A.

2. A method of producing the plant of claim 1 , the method comprising the steps of:
(a) crossing first and second soybean plants, wherein the first and second plants collectively comprise a GmSHMT allele containing mutations P130R and N358Y resulting in an SCN resistant phenotype, and wherein the first and second plants collectively comprise germplasm capable of conferring agronomically elite characteristics to a progeny plant of said plants;
(b) assaying progeny soybean plants resulting from the crossing for agronomically elite characteristics and for SCN resistance; and
(c) selecting at least a first progeny plant comprising said SCN resistant phenotype and agronomically elite characteristics.

3. A plant part of the plant of claim 1 .

Assignees

• Meksem Khalid
• Liu Shiming
• Kandoth Pramod Kaitheri
• Mitchum Melissa G
• Board Of Trustees Of Southern Illinois Univ
• Univ Missouri

Inventors

• Meksem Khalid
• Liu Shiming
• Kandoth Pramod Kaitheri
• Mitchum Melissa G

Classifications

• C12N15/8218

  Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS] · CPC title

• A01H1/04

  Processes of selection {involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection} · CPC title

• C12N15/8227

  Root-specific · CPC title

• A01H6/542 Primary

  Glycine max [soybean] · CPC title

• C12N15/8285

  for nematode resistance · CPC title