Acyl-ACP thioesterase
US-9828613-B2 · Nov 28, 2017 · US
Method of producing lipid by using β-ketoacyl-ACP synthase
US10066248B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10066248-B2 |
| Application number | US-201515110635-A |
| Country | US |
| Kind code | B2 |
| Filing date | Feb 23, 2015 |
| Priority date | Mar 3, 2014 |
| Publication date | Sep 4, 2018 |
| Grant date | Sep 4, 2018 |
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- Title
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Abstract
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The present disclosure provides a gene encoding a protein having β-ketoacyl-ACP synthase activity having at least 60% or more identity with the amino acid sequence set forth in SEQ ID NO:1, the protein encoded by the gene, a method of producing a transformant by transforming a host with the gene, a transformant that is transformed with the gene, and a method of producing a lipid, especially, a lipid containing a medium chain fatty acid or ester thereof, by culturing the transformed host that expresses the gene. In some embodiments, the transformant also has a gene encoding an acyl-ACP thioesterase.
First claim
Opening claim text (preview).
What is claimed is: 1. A method of producing a lipid, comprising the following steps (1) and (2): (1) transforming a host cell with (i) a gene encoding the following protein (a) or (b) and (ii) a gene encoding an acyl-ACP thioesterase having specificity for a medium chain acyl-ACP, thereby obtaining a transformant, and (2) allowing the transformant to produce a lipid and collecting the lipid from the transformant; wherein (a) and (b) are: (a) A protein consisting of the amino acid sequence set forth in SEQ ID NO: 1; and (b) A protein consisting of an amino acid sequence having 95% or more sequence identity with the amino acid sequence of SEQ ID NO: 1, and having β-ketoacyl-ACP synthase activity; wherein the host cell is a microalga. 2. The method of producing a lipid according to claim 1 , wherein the lipid is a medium chain fatty acid or an ester thereof. 3. The method of producing a lipid according to claim 1 , wherein the protein (b) is a β-ketoacyl-ACP synthase having specificity for a medium chain acyl-ACP. 4. The method of producing a lipid according to claim 2 , wherein the medium-chain fatty acid is a C12:0 or C14:0 fatty acid or an ester thereof. 5. The method of producing a lipid according to claim 1 , wherein step (2) comprises culturing the transformant in a cerulenin-containing medium. 6. The method of producing a lipid according to claim 4 , wherein the medium chain fatty acid is a C12:0 fatty acid or an ester thereof. 7. The method of producing a lipid according to claim 1 , wherein the microalga belongs to the genus Nannochloropsis. 8. The method of producing a lipid according to claim 4 , wherein the medium chain fatty acid is a C14:0 fatty acid or an ester thereof. 9. A host cell transformed with (i) a gene encoding the following protein (a) or (b) and (ii) a gene encoding an acyl-ACP thioesterase having specificity for a medium chain acyl-ACP; wherein (a) and (b) are: (a) A protein consisting of the amino acid sequence set forth in SEQ ID NO: 1; and (b) A protein consisting of an amino acid sequence having 95% or more sequence identity with the amino acid sequence of SEQ ID NO: 1, and having β-ketoacyl-ACP synthase activity; wherein the host cell is a microalga. 10. The transformed host cell according to claim 9 , wherein the transformed host cell produces a C12:0 or C14:0 fatty acid or an ester thereof. 11. The transformed host cell according to claim 10 , wherein the transformed host cell produces a C12:0 fatty acid or an ester thereof. 12. The transformed host cell according to claim 9 , wherein the microalga belongs to the genus Nannochloropsis. 13. The transformed host cell according to claim 10 , wherein the transformed host cell produces a C14:0 fatty acid or an ester thereof.
Assignees
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Classifications
- C12P7/6436Primary
Fatty acid esters · CPC title
Vectors or expression systems specially adapted for E. coli · CPC title
Lyases (4.) · CPC title
Oleoyl-[acyl-carrier-protein] hydrolase (3.1.2.14), i.e. ACP-thioesterase · CPC title
Vectors or expression systems specially adapted for eukaryotic hosts · CPC title
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- What does patent US10066248B2 cover?
- The present disclosure provides a gene encoding a protein having β-ketoacyl-ACP synthase activity having at least 60% or more identity with the amino acid sequence set forth in SEQ ID NO:1, the protein encoded by the gene, a method of producing a transformant by transforming a host with the gene, a transformant that is transformed with the gene, and a method of producing a lipid, especially, a …
- Who is the assignee on this patent?
- Kao Corp
- What technology area does this patent fall under?
- Primary CPC classification C12P7/6436. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Sep 04 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 7 related publications on this page (citations in our corpus or others sharing the same primary CPC).