Substrate-selective co-fermentation process
US-9212346-B2 · Dec 15, 2015 · US
Yeast cells having NADP(H)-dependent reductive TCA pathway from pyruvate to succinate
US10066246B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10066246-B2 |
| Application number | US-201314416631-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 25, 2013 |
| Priority date | Jul 25, 2012 |
| Publication date | Sep 4, 2018 |
| Grant date | Sep 4, 2018 |
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Abstract
Official abstract text for this publication.
Recombinant yeast cells contain a reductive TCA pathway from phosphoenolpyruvate or pyruvate to succinate. At least one metabolic step in the pathway includes a reaction of NADPH to produce NADP+. The yeast cell contains at least one exogenous NADPH-dependent gene in the pathway from phosphoenolpyruvate or pyruvate to succinate, preferably an NADPH-dependent malate dehydrogensase or fumarate reducase gene (or both).
First claim
Opening claim text (preview).
The invention claimed is: 1. A recombinant I. orientalis cell having an active reductive TCA pathway from pyruvate to succinate and which further metabolizes succinate to one or more succinate metabolization products, which reductive TCA pathway includes a reaction that oxidizes NADPH to NADP+ comprising a conversion of fumarate to succinate catalyzed by an NADPH-dependent fumarate reductase enzyme and the recombinant cell has integrated into its genome an heterologous fumarate reductase gene that encodes for the NADPH-dependent fumarate reductase enzyme. 2. The recombinant cell of claim 1 , wherein the recombinant cell further includes a reaction that oxidizes NADPH to NADP+comprising a conversion of oxaloacetate to malate catalyzed by an NADPH-dependent malate dehydrogenase enzyme and the recombinant cell has integrated into its genome an heterologous malate dehydrogenase gene that encodes for the NADPH-dependent malate dehydrogenase enzyme. 3. The recombinant cell of claim 1 wherein the recombinant cell further includes a step of converting pyruvate or phosphoenolpyruvate to oxaloacetate, a step of converting oxaloacetate to malate, and a step of converting malate to fumarate. 4. The recombinant I. orientalis cell of claim 1 , wherein the succinate metabolization product is one or more of 1,4-butanediol, 1,3-butadiene, propionic acid, and 3-hydroxyisobutryic acid.
Assignees
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Classifications
from Saccharomyces · CPC title
- C12P7/46Primary
Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid · CPC title
Lyases (4.) · CPC title
Pyruvate carboxylase (6.4.1.1) · CPC title
acting on CH-OH groups as donors (1.1) · CPC title
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- What does patent US10066246B2 cover?
- Recombinant yeast cells contain a reductive TCA pathway from phosphoenolpyruvate or pyruvate to succinate. At least one metabolic step in the pathway includes a reaction of NADPH to produce NADP+. The yeast cell contains at least one exogenous NADPH-dependent gene in the pathway from phosphoenolpyruvate or pyruvate to succinate, preferably an NADPH-dependent malate dehydrogensase or fumarate re…
- Who is the assignee on this patent?
- Cargill Inc, Bioamber Sas, Cargill Inc
- What technology area does this patent fall under?
- Primary CPC classification C12P7/46. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Sep 04 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).