Amylases, nucleic acids encoding them and method of producing an oil

Patent metadata

Field	Value
Publication number	US-10066222-B2
Application number	US-201715636039-A
Country	US
Kind code	B2
Filing date	Jun 28, 2017
Priority date	Feb 21, 2001
Publication date	Sep 4, 2018
Grant date	Sep 4, 2018

Abstract

In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of starch into sugars.

First claim

What is claimed is:

1. A method for producing an oil from a grain milling process comprising:
(a) providing at least one alpha-amylase having at least 96% sequence identity to the amino acid sequence as set forth in SEQ ID NO:2;
(b) providing a composition comprising a starch;
(c) contacting the alpha-amylase of (a) with the composition of (b) under conditions wherein the alpha-amylase liquefies the starch to produce a water soluble starch solution
(d) removing the solubles from the starch solution to produce the oil.

2. The method of claim 1 , wherein the starch is a corn starch.

3. The method of claim 1 , wherein the oil is endogenous to the corn.

4. The method of claim 1 , wherein the removal or liquefaction of starch reduces viscosity of the water soluble starch solution.

5. The method of claim 4 , wherein the reduction in viscosity improves the amount of oil produced.

6. The method of claim 5 , wherein the alpha-amylase is thermostable at a temperature from about 37° C. to about 105° C.

7. The method of any of claim 1 , wherein the alpha-amylase is active at low pH.

8. The method of claim 1 , wherein the alpha-amylase is active at about pH 4 to about pH 6.

Assignees

• Basf Enzymes Llc

Inventors

• Callen Walter
• Richardson Toby
• Frey Gerhard
• Miller Carl A
• Kazaoka Martin
• Mathur Eric J
• Short Jay M

Classifications

• C12N9/2417

  from microbiological source · CPC title

• C12P19/14

  produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title

• C12N9/2411 Primary

  Amylases · CPC title