Method for preparing induced pluripotent stem cells using synthetic peptide

Patent metadata

Field	Value
Publication number	US-10066212-B2
Application number	US-201615514337-A
Country	US
Kind code	B2
Filing date	Jul 19, 2016
Priority date	Aug 6, 2015
Publication date	Sep 4, 2018
Grant date	Sep 4, 2018

Abstract

Provided is a method of preparing induced pluripotent stem cells using a synthetic peptide, and more particularly, to a method of preparing induced pluripotent stem cells using a peptide capable of inhibiting the activity of NF-κB and promoting mesenchymal-epithelial transition (MET). Since undifferentiated multipotent stem cells may be efficiently prepared under xenopathogen-free or feeder cell-free conditions without requiring co-culture with animal serum or xenogeneic cells, the method for preparing the induced pluripotent stem cells using the synthetic peptide according to the present disclosure is very useful for developing stem cell therapeutic agents that are clinically applicable.

First claim

What is claimed is:

1. A method for preparing induced pluripotent stem (iPS) cells, the method comprising:
(a) introducing one or more nucleic acid sequences encoding reprogramming factors Oct3/4, Sox2 and Klf4 into isolated mammalian somatic cells and then culturing; and
(b) introducing a peptide having any one of amino acid sequence selected from the group consisting of SEQ ID NOs: 1 to 3 into the isolated mammalian somatic cells to which the reprogramming factor gene has been introduced and then culturing, such that iPS cells are obtained.

2. The method of claim 1 , wherein the peptide has a concentration of 0.01 to 100 μM.

3. The method of claim 1 , wherein the somatic cells are human somatic cells.

4. The method of claim 1 , wherein the iPS cells are prepared under xenopathogen-free or feeder cell-free conditions.

5. The method of claim 1 , wherein the reprogramming factor further comprises c-Myc or Lin28.

6. The method of claim 1 , wherein step b) comprises introducing the peptide in the presence of a biomaterial.

7. The method of claim 6 , wherein the biomaterial is a synthetic polymer or a natural polymer.

8. The method of claim 7 , wherein the synthetic polymer is any one selected form the group consisting of poloxamer, polyethylene glycol and polypropylene glycol.

9. The method of claim 7 , wherein the natural polymer is any one selected from vitronectin, collagen, gelatin, alginic acid, chondroitin sulfate, fibronectin and an extracellular matrix protein.

Assignees

• Seoul Nat Univ R&Db Foundation
• Nano Intelligent Biomedical Eng Corporation Co Ltd

Inventors

• Park Yoon Jeong
• Chung Chong-Pyoung
• Suh Jin Sook
• Lee Jue-Yeon
• Lee Dong Woo

Classifications

• C07K14/4703

  Inhibitors; Suppressors · CPC title

• C07K14/00

  Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof · CPC title

• C12N5/005

  Protein-free medium · CPC title

• C12N2501/998

  Proteins not provided for elsewhere · CPC title

• C07K14/4723

  Cationic antimicrobial peptides, e.g. defensins · CPC title