Polypeptide porous body and method for producing same

Patent metadata

Field	Value
Publication number	US-10065997-B2
Application number	US-201414764463-A
Country	US
Kind code	B2
Filing date	Apr 18, 2014
Priority date	Apr 25, 2013
Publication date	Sep 4, 2018
Grant date	Sep 4, 2018

Abstract

A polypeptide porous body of the present invention is a porous body of a polypeptide derived from spider silk proteins. The polypeptide includes a water-insoluble polypeptide. The polypeptide porous body has an apparent density of 0.1 g/cm 3 or less. A method for producing the polypeptide porous body includes: a solution production step in which the polypeptide is dissolved in at least one solvent selected from DMSO, DMF, and these with an inorganic salt, so as to obtain a solution of the polypeptide; a step in which the solution produced in the solution production step is substituted with a water-soluble solvent so as to obtain a polypeptide gel; and a step in which the polypeptide gel is dried. Thereby, the present invention provides a polypeptide porous body having excellent water absorbability and a polypeptide porous body suitable for application to a living body, and a method for producing the same.

First claim

The invention claimed is:

1. A polypeptide porous body that is a porous body of a polypeptide derived from spider silk proteins,
wherein the polypeptide includes a water-insoluble polypeptide derived from spider silk proteins,
the polypeptide porous body has an apparent density of 0.1 g/cm 3 or less, and
at least one selected from the group consisting of dimethyl sulfoxide and N, N-dimethylformamide is present inside the polypeptide porous body.

2. The polypeptide porous body according to claim 1 , wherein the polypeptide porous body has an apparent density of 0.01 to 0.08 g/cm 3 .

3. The polypeptide porous body according to claim 1 , wherein the polypeptide porous body has a water absorption ratio of 2 to 20 times in terms of weight of the polypeptide porous body.

4. A method for producing a polypeptide porous body, comprising:
a solution production step in which a polypeptide derived from spider silk proteins is dissolved in at least one dissolving solvent selected from the group consisting of: (A) dimethyl sulfoxide; (B) dimethyl sulfoxide with an inorganic salt; and (C) N, N-dimethylformamide with an inorganic salt, so as to obtain a solution of the polypeptide;
a step in which the solution of the polypeptide produced in the solution production step is substituted with a water-soluble solvent so as to obtain a polypeptide gel; and
a step in which the polypeptide gel is dried.

5. The method for producing a polypeptide porous body according to claim 4 , wherein the polypeptide porous body is produced using an aqueous solution that is obtained by substituting the solution of the polypeptide with water.

6. The method for producing a polypeptide porous body according to claim 4 ,
wherein the substitution step using the water-soluble solvent is a step in which the solution of the polypeptide obtained by dissolving the polypeptide in the dissolving solvent is placed in a dialysis membrane,
the dialysis membrane is immersed in a water-soluble solvent, and
the water-soluble solvent is renewed at least one time.

7. The method for producing a polypeptide porous body according to claim 4 , wherein the drying is vacuum freeze-drying.

Assignees

• Spiber Inc

Inventors

• Osawa Toshiaki
• Morita Keisuke

Classifications

• C08J9/28

  by elimination of a liquid phase from a macromolecular composition or article, e.g. drying of coagulum · CPC title

• A61K8/42

  Amides · CPC title

• B01J20/24

  Naturally occurring macromolecular compounds, e.g. humic acids or their derivatives · CPC title

• A61Q19/00

  Preparations for care of the skin · CPC title

• C08J2205/028

  Xerogel, i.e. an air dried gel · CPC title