Therapeutic and diagnostic probes

Patent metadata

Field	Value
Publication number	US-10064943-B2
Application number	US-201514728247-A
Country	US
Kind code	B2
Filing date	Jun 2, 2015
Priority date	Jun 2, 2014
Publication date	Sep 4, 2018
Grant date	Sep 4, 2018

Abstract

The present invention provides compositions and methods of use of nanoparticle-based probes for in vivo imaging and therapy. The probes can be used to track diseased target cells by non-invasive imaging in the near-infrared range. Additionally, the probes can induce cell death of the target cells via photodynamic treatment.

First claim

What is claimed is:

1. A probe comprising a nanocarrier attached to both (1) an imaging agent, wherein the imaging agent is a cyanine dye; and (2) a therapeutic agent, which is IRDYE700DX, wherein said nanocarrier is an antibody or a fragment thereof.

2. The probe of claim 1 , wherein said therapeutic agent is attached to said nanocarrier via a first linker.

3. The probe of claim 2 , wherein said imaging agent is attached to said nanocarrier via a second linker.

4. A method for detecting a target cell in an individual, said method comprising:
(a) contacting said target cell with a probe comprising a nanocarrier attached to both (1) an imaging agent, which imaging agent is a cyanine dye; and (2) a therapeutic agent, wherein the therapeutic agent is IRDYE700DX, wherein the probe selectively associates with the said target cell, wherein said nanocarrier is an antibody or fragment thereof; and
(b) detecting said imaging agent thereby providing an indication of the presence and/or location of said target cell in the individual.

5. The method of claim 4 , wherein said contacting comprises systemic administration to the individual, local administration to a tumor, or administration to a surgical site.

6. The method of claim 4 , wherein said target cell is a cell of a solid tumor.

7. The method claim 4 , further comprising exposing said therapeutic agent to a photoactivating light, thereby inducing apoptosis and/or necrosis of said target cell in the individual.

8. A method for inducing cell death in a target cell in an individual, said method comprising:
(a) contacting said target cell with a probe comprising a nanocarrier attached to both (1) an imaging agent, which imaging agent is a cyanine dye; and (2) a therapeutic agent, wherein the therapeutic agent is IRDYE700DX, wherein the probe selectively associates with said target cell, wherein said nanocarrier is an antibody or fragment thereof; and
(b) exposing said therapeutic agent to a photoactivating light, thereby inducing apoptosis and/or necrosis of said target cell in the individual.

9. The method of claim 8 , wherein said contacting comprises systemic administration to the individual, local administration to a tumor, or administration to a surgical site.

Assignees

• Li Cor Inc

Inventors

• Dilley David L
• Kovar Joy

Classifications

• A61P9/00

  Drugs for disorders of the cardiovascular system · CPC title

• A61P43/00

  Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title

• A61P9/10

  for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis · CPC title

• A61P35/00

  Antineoplastic agents · CPC title

• A61P31/04

  Antibacterial agents · CPC title