Mesenchymal stem cell differentiation

Patent metadata

Field	Value
Publication number	US-10064918-B2
Application number	US-201514859126-A
Country	US
Kind code	B2
Filing date	Sep 18, 2015
Priority date	Jul 14, 2009
Publication date	Sep 4, 2018
Grant date	Sep 4, 2018

Abstract

The present invention provides for methods and compositions for treating or preventing arthritis and joint injury.

First claim

What is claimed is:

1. A polypeptide consisting of SEQ ID NO: 5, wherein the polypeptide is PEGylated.

2. A pharmaceutical composition comprising an effective amount of the polypeptide of claim 1 .

3. The pharmaceutical composition of claim 2 , further comprising a buffer.

4. The pharmaceutical composition of claim 2 , further comprising hyaluronic acid.

5. The pharmaceutical composition of claim 2 , wherein the pharmaceutical composition is suitable for intra-articular delivery.

6. A pharmaceutical composition comprising a polypeptide consisting of SEQ ID NO: 5, and a preservative.

7. The polypeptide of claim 1 , wherein the polypeptide has chondrogenic activity and reduced ability to increase triglyceridemia.

8. The polypeptide of claim 1 , wherein the polypeptide has reduced heterogeneous glycosylation relative to a naturally-occurring human ANGPTL3 protein.

9. A fusion protein comprising a polypeptide consisting of SEQ ID NO: 5 fused to a human serum albumin (HSA), an immunoglobulin heavy chain constant region (Fc), a polyhistidine, a glutathione S transferase (GST), a thioredoxin, a protein A, a protein G, or a maltose binding protein (MBP).

10. A pharmaceutical composition comprising an effective amount of the fusion protein of claim 9 .

11. The pharmaceutical composition of claim 10 , further comprising a buffer.

12. The pharmaceutical composition of claim 10 , further comprising hyaluronic acid.

13. The pharmaceutical composition of claim 10 , wherein the pharmaceutical composition is suitable for intra-articular delivery.

Assignees

• Jennings Lori
• Johnson Kristen
• Schultz Peter
• Novartis Ag

Inventors

• Jennings Lori
• Johnson Kristen
• Schultz Peter

Classifications

• A61P43/00

  Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title

• A61P29/00

  Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID] · CPC title

• A61P19/02

  for joint disorders, e.g. arthritis, arthrosis · CPC title

• A61P19/00

  Drugs for skeletal disorders · CPC title

• A61K38/00

  Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) · CPC title