Automated embedding machine, and methods for embedding a histological sample

Patent metadata

Field	Value
Publication number	US-10060839-B2
Application number	US-201715499314-A
Country	US
Kind code	B2
Filing date	Apr 27, 2017
Priority date	Dec 10, 2013
Publication date	Aug 28, 2018
Grant date	Aug 28, 2018

Abstract

An automated embedding machine includes at least a conveyor, a pouring station, and a cooling station. The conveyor is embodied and arranged to receive casting molds each having at least one histological sample arranged therein, and to transport them to the pouring station which fills each casting mold with an embedding medium heated to above its melting point, and then to the cooling station which cools the embedding medium of each casting mold to below its melting point. The disclosure furthermore relates to a method for embedding a histological sample.

First claim

What is claimed is:

1. A method for embedding histological samples, comprising the steps of:
a. inserting histological samples into casting molds, at least one of the histological samples being inserted into one of the casting molds, wherein the samples are each aligned relative to the respective casting mold in a target position in which the samples are to be block-embedded, and are secured in the target position with a securing element;
b. transferring the casting molds having the samples to a loading station of an automated embedding machine, the automated embedding machine further comprising a conveyor, a pouring station, and a cooling station, wherein the loading station is embodied as a loading buffer configured to hold a plurality of the casting molds;
c. conveying the transferred casting molds, using the conveyor, to the pouring station that fills each of the casting molds with an embedding medium heated to above a melting point of the embedding medium; and
d. conveying the casting molds, filled with the embedding medium, to the cooling station that cools the embedding medium of each of the casting molds to below the melting point of the embedding medium, wherein the securing element is removed from the sample while the embedding medium is cooled.

2. The method according to claim 1 , wherein the securing element is removed from the sample by magnetic force.

3. The method according to claim 1 , wherein the automated embedding machine comprises at least one further station, and the casting molds are transported by the conveyor to the further station.

4. The method according to claim 1 , wherein the conveyor receives the casting molds for further transport from the loading station.

5. The method according to claim 1 , further comprising the step of identifying each of the casting molds and/or the at least one sample present therein and/or each solidified block by reading or receiving a specific code associated with the casting mold and/or the at least one sample and/or the solidified block.

6. The method according to claim 5 , further comprising the step of outputting an information item about each of the casting molds and/or the at least one sample present therein and/or each solidified block to a laboratory information system.

7. The method according to claim 1 , wherein the casting molds are transported in a continuous transport stream along a predefined conveying path.

8. The method according to claim 1 , wherein the casting molds are transported individually along a predefined transport path.

9. The method according to claim 1 , wherein the casting molds are transported in groups along a predefined conveying path.

10. The method according to claim 9 , wherein the casting molds of each of the groups are secured in a shared holder.

11. The method according to claim 1 , wherein the conveyor comprises a holder for multiple casting molds, the holder being movable relative to the loading, pouring, and cooling stations by a control apparatus such that each of the casting molds held by the holder travels successively to each of the loading, pouring, and cooling stations.

12. The method according to claim 11 , wherein the conveying path is identical for all the casting molds.

13. The method according to claim 1 , further comprising transporting the casting molds having the cooled embedding medium by the conveyor to an unloading station of the automated embedding machine, wherein the unloading station is embodied as an unloading buffer configured to hold a plurality of the casting molds.

Assignees

• Leica Biosystems Nussloch Gmbh

Inventors

• Berberich Markus

Classifications

• G01N2001/366

  Moulds; Demoulding · CPC title

• G01N1/36 Primary

  Embedding or analogous mounting of samples · CPC title