Molecular markers associated with soybean tolerance to low iron growth conditions

Patent metadata

Field	Value
Publication number	US-10059999-B2
Application number	US-201414300384-A
Country	US
Kind code	B2
Filing date	Jun 10, 2014
Priority date	Jun 10, 2013
Publication date	Aug 28, 2018
Grant date	Aug 28, 2018

Abstract

The present invention provides methods and compositions for identifying soybean plants that are tolerant or have improved tolerance, or those that are susceptible to, iron deficient growth conditions. The methods use molecular markers to identify, select, and/or introgress genetic loci modulating phenotypic expression of an iron deficiency tolerance trait in soybean plant breeding. Methods are provided for screening germplasm entries for the performance and expression of this trait.

First claim

What is claimed is:

1. A method of creating a population of soybean plants with a low iron growth condition tolerant phenotype, said method comprising:
a. genotyping a first population of soybean plants or seeds;
b. detecting in said first population of soybean plants or seeds, a polymorphic nucleic acid marker linked by less than or equal to 10 cM to an allele of SEQ ID NO:70, wherein said allele of SEQ ID NO:70 is associated with a low iron growth condition tolerant phenotype;
c. selecting based upon said genotyping a soybean plant or seed containing said allele that was detected by genotyping;
d. crossing or selfing said selected soybean plant or a plant produced from said selected seed; and
e. producing from said crossing or selfing a population of progeny plants comprising said allele of SEQ ID NO:70 that is associated with the low iron growth condition tolerant phenotype;
wherein said allele of SEQ ID NO:70 comprises an Adenosine at position 201.

2. A method of claim 1 , wherein said marker locus is genetically linked by less than or equal to 5 cM to SEQ ID NO:70.

3. The method of claim 1 , wherein said marker locus is in a genomic region flanked by SEQ ID NO:70 and SEQ ID NO:74.

4. The method of claim 1 , wherein said marker locus is genetically linked by less than or equal to 1 cM to SEQ ID NO:70.

5. The method of claim 1 , wherein said marker locus is from a chromosome region flanked by any two of marker loci selected from the group consisting of SEQ ID NOs: 70 to 74.

6. The method of claim 1 , wherein said marker locus is of a marker type selected from the group consisting of Restriction Fragment Length Polymorphisms (RFLP), Amplified Fragment Length Polymorphisms (AFLP), Simple Sequence Repeats (SSR), Single Nucleotide Polymorphisms (SNP), Insertion/Deletion Polymorphisms (Indels), Variable Number Tandem Repeats (VNTR), and Random Amplified Polymorphic DNA (RAPD).

7. The method of claim 1 , wherein said detecting an allele comprising a method selected form the group consisting of a PCR-based detection method, a microarray method, and a nucleic acid sequencing method.

8. The method of claim 1 , wherein said detecting an allele comprising a single base extension (SBE) assay.

Assignees

• Monsanto Technology Llc

Inventors

• Jia Hongwu
• La Vallee Bradley
• Lussenden Roger L
• Yates Jennifer L
• Ye Xianghai

Classifications

• Y10T436/143333

  Saccharide [e.g., DNA, etc.] · CPC title

• C12Q2600/13

  Plant traits · CPC title

• C12Q1/6895 Primary

  for plants, fungi or algae · CPC title

• A01H1/04

  Processes of selection {involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection} · CPC title

• A01H1/045

  using molecular markers · CPC title