Photonic superlattice-based devices and compositions for use in luminescent imaging, and methods of using the same

Patent metadata

Field	Value
Publication number	US-10059992-B2
Application number	US-201715468024-A
Country	US
Kind code	B2
Filing date	Mar 23, 2017
Priority date	Mar 24, 2016
Publication date	Aug 28, 2018
Grant date	Aug 28, 2018

Abstract

Under one aspect, a device is provided for use in luminescent imaging. The device can include a photonic superlattice including a first material, the first material having a first refractive index. The first material can include first and second major surfaces and first and second pluralities of features defined though at least one of the first and second major surfaces, the features of the first plurality differing in at least one characteristic from the features of the second plurality. The photonic superlattice can support propagation of a first wavelength and a second wavelength approximately at a first angle out of the photonic superlattice, the first and second wavelengths being separated from one another by a first non-propagating wavelength that does not selectively propagate at the first angle out of the photonic superlattice. The device further can include a second material having a second refractive index that is different than the first refractive index. The second material can be disposed within, between, or over the first and second pluralities of features and can include first and second luminophores. The device further can include a first optical component disposed over one of the first and second major surfaces of the first material. The first optical component can receive luminescence emitted by the first luminophore at the first wavelength approximately at the first angle, and can receive luminescence emitted by the second luminophore at the second wavelength approximately at the first angle.

First claim

What is claimed:

1. A device for use in luminescent imaging, the device comprising:
a photonic superlattice comprising a first material,
the first material having a first refractive index,
the first material comprising first and second major surfaces and first and second pluralities of features defined though at least one of the first and second major surfaces,
the features of the first plurality differing in at least one characteristic from the features of the second plurality; and
the photonic superlattice supporting propagation of a first wavelength and a second wavelength approximately at a first angle out of the photonic superlattice, the first and second wavelengths being separated from one another by a first non-propagating wavelength that does not selectively propagate at the first angle out of the photonic superlattice;

a second material having a second refractive index that is different than the first refractive index,
the second material being disposed within, between, or over the first and second pluralities of features and comprising first and second luminophores; and

a first optical component disposed over one of the first and second major surfaces of the first material,
the first optical component receiving luminescence emitted by the first luminophore at the first wavelength approximately at the first angle, and
the first optical component receiving luminescence emitted by the second luminophore at the second wavelength approximately at the first angle.

2. The device of claim 1 , wherein the first and second pluralities of features respectively comprise first and second pluralities of wells.

3. The device of claim 1 , the second material further comprising third and fourth luminophores,
the photonic superlattice further supporting propagation of a third wavelength and a fourth wavelength approximately at the first angle out of the photonic superlattice, the third and fourth wavelengths being separated from one another by a second non-propagating wavelength that does not selectively propagate at the first angle,
the optical component receiving luminescence emitted by the third luminophore at the third wavelength approximately at the first angle, and
the optical component receiving luminescence emitted by the fourth luminophore at the fourth wavelength approximately at the first angle.

4. The device of claim 3 , wherein the first luminophore is coupled to a first nucleic acid, the second luminophore is coupled to a second nucleic acid that is different than the first nucleic acid, the third luminophore is coupled to a third nucleic acid that is different than the first and second nucleic acids, and the fourth luminophore is coupled to a fourth nucleic acid that is different than the first, second, and third nucleic acids.

5. The device of claim 1 , comprising a second optical component configured so as to transmit radiation to the photonic superlattice approximately at a second angle,
the first luminophore emitting the first wavelength responsive to the radiation transmitted by the second optical component, and
the second luminophore emitting the second wavelength responsive to the radiation transmitted by the second optical component.

6. The device of claim 5 , wherein the second angle is approximately the same as the first angle or wherein the second angle is approximately orthogonal to the first angle.

7. The device of claim 5 , wherein the first optical component is disposed over the first major surface of the first material, and wherein the second optical component is disposed over the second major surface of the first material.

8. The device of claim 5 , further comprising a broadband excitation source configured to generate the radiation transmitted to the photonic superlattice by the second optical component.

9. The device of claim 1 , further comprising at least one microfluidic feature in contact with the photonic superlattice and configured to provide a flow of one or more analytes to the first and second pluralities of features.

10. The device of claim 1 , wherein the first optical component comprises an image sensor configured to image the received first and second wavelengths.

11. The device of claim 1 , wherein the first material comprises a polymer or a glass, and/or wherein the second material comprises a fluid or a gel.

12. The device of claim 1 , wherein the first luminophore is coupled to a first polynucleotide to be sequenced, and the second luminophore is coupled to a second polynucleotide to be sequenced.

13. The device of claim 12 , further comprising:
a first polymerase adding a first nucleic acid to a third polynucleotide that is complementary to and coupled to the first polynucleotide, the first nucleic acid being coupled to the first luminophore; and
a second polymerase adding a second nucleic acid to a fourth polynucleotide that is complementary to and coupled to the second polynucleotide, the second nucleic acid being coupled to the second luminophore.

14. The device of claim 13 , further comprising a channel flowing a first liquid comprising the first and second nucleic acids and the first and second polymerases into, between, or over the first and second pluralities of features.

15. A method for use in luminescent imaging, the method comprising:
providing a photonic superlattice comprising a first material,
the first material having a first refractive index,
the first material comprising first and second major surfaces and first and second pluralities of features defined though at least one of the first and second major surfaces,
the features of the first plurality differing in at least one characteristic from the features of the second plurality;
the photonic superlattice supporting propagation of a first wavelength and a second wavelength approximately at a first angle out of the photonic superlattice, the first and second wavelengths being separated from one another by a first non-propagating wavelength that does not selectively propagate at the first angle out of the photonic superlattice;

providing a second material having a second refractive index that is different than the first refractive index,
the second material being disposed within, between, or over the first and second pluralities of features and comprising first and second luminophores;

providing a first optical component disposed over one of the first and second major surfaces of the first material;
receiving by the first optical component luminescence emitted by the first luminophore at the first wavelength approximately at the first angle; and
receiving by the first optical component luminescence emitted by the second luminophore at the second wavelength approximately at the first angle.

16. The method of claim 15 , the second material further comprising third and fourth luminophores,
the photonic superlattice further supporting propagation of a third wavelength and a fourth wavelength approximately at the first angle out of the photonic superlattice, the third and fourth wavelengths being different than each of the first and second wavelengths and being separated from one another by a second non-propagating wavelength that does not selectively propagate at the first angle, the method further comprising:
receiving by the first optical component luminescence emitted by the third luminophore at the third wavelength approximately at the first angle; and
receiving by the first optical component luminescence emitted by the fourth luminophore at the fourth wavelength approximately at the first angle.

17. The method of claim 15 ,

Assignees

• Illumina Inc

Inventors

• Dehlinger Dietrich
• Zhong Cheng Frank
• Topolancik Juraj

Classifications

• G02B1/005

  made of photonic crystals or photonic band gap materials (photonic band-gap structures or photonic lattices in integrated optics G02B6/1225; photonic band-gap structures or photonic lattices in optical fibres G02B6/02295) · CPC title

• G01N21/6486 Primary

  Measuring fluorescence of biological material, e.g. DNA, RNA, cells (G01N21/6428 takes precedence) · CPC title

• G01N21/6452 Primary

  Individual samples arranged in a regular 2D-array, e.g. multiwell plates · CPC title

• C12Q1/6874 Primary

  involving nucleic acid arrays, e.g. sequencing by hybridisation · CPC title

• G01N21/01

  Arrangements or apparatus for facilitating the optical investigation · CPC title