Nanostructured spore carrier
US-9650661-B2 · May 16, 2017 · US
US10059977B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10059977-B2 |
| Application number | US-201715484781-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 11, 2017 |
| Priority date | May 21, 2013 |
| Publication date | Aug 28, 2018 |
| Grant date | Aug 28, 2018 |
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Spore carriers suitable for use in biological indicators are described. The spore carriers include a substrate such as polymeric film or non-woven web with a hydrophilic nanostructured layer bonded to it. Spores are bonded to the nanostructured layer. Nanostructured layers including nanoparticles, such as acid-sintered silica nanoparticles are described. Biological indicators including such spore carriers are also described.
Opening claim text (preview).
What is claimed is: 1. A biological sterilization indicator comprising: a housing defining a reservoir, the housing comprising a cap that includes one or more apertures to provide fluid communication between the reservoir and ambience; a frangible container within the housing; and a spore carrier comprising a substrate, a hydrophilic nanostructured layer bonded to a surface of the substrate and spores bonded to the nanostructured layer, wherein the nanostructured layer comprises silica nanoparticles. 2. The biological sterilization indicator of claim 1 , wherein the nanostructured layer has a receding contact angle of no greater than 50 degrees as measured by the Contact Angle Method. 3. The biological sterilization indicator of claim 1 , wherein the nanostructured layer has a receding contact angle of no greater than 20 degrees as measured by the Contact Angle Method. 4. The biological sterilization indicator of claim 1 , wherein the frangible container contains liquid. 5. The biological sterilization indicator of claim 4 , wherein the liquid comprises nutrient medium. 6. The biological sterilization indicator of claim 1 , wherein the silica nanoparticles are acid sintered. 7. The biological sterilization indicator of claim 1 , wherein the nanoparticles have an average diameter of no greater than 20 nm. 8. The biological sterilization indicator of claim 1 , wherein the nanostructured layer has an RMS roughness of at least 10 nm. 9. The biological sterilization indicator of claim 8 , the nanostructured layer has an RMS roughness of no greater than 500 nm. 10. The biological sterilization indicator of claim 1 , wherein the nanostructured layer further comprises microparticles. 11. The biological sterilization indicator of claim 1 , wherein the nanostructured layer further comprises a binder. 12. The biological sterilization indicator of claim 11 , wherein the binder comprises a tetraalkoxysilane. 13. The biological sterilization indicator of claim 1 , wherein the substrate is a polymeric substrate. 14. The biological sterilization indicator of claim 13 , wherein the polymeric substrate comprises polyester. 15. The biological sterilization indicator of claim 13 , wherein the polymeric substrate comprises polypropylene. 16. The biological sterilization indicator of claim 1 , wherein the substrate is a woven or non-woven web. 17. The biological sterilization indicator of claim 1 , wherein the substrate is a film. 18. The biological sterilization indicator of claim 1 , wherein the spores comprise at least one of Geobacillus stearothermophilus and Bacillus atrophaeus. 19. The biological sterilization indicator of claim 1 , wherein the spore carrier further comprises one or more wells. 20. The biological sterilization indicator of claim 1 , wherein the nanostructured layer comprises silica nanoparticles deposited out of an aqueous coating composition having a pH of less than 3.
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