Glycosyltransferase gene and use thereof

Patent metadata

Field	Value
Publication number	US-10059956-B2
Application number	US-201314372232-A
Country	US
Kind code	B2
Filing date	Jan 16, 2013
Priority date	Jan 17, 2012
Publication date	Aug 28, 2018
Grant date	Aug 28, 2018

Abstract

Provided is a polynucleotide encoding a protein having activity that transfers a sugar to the hydroxyl group at the 4′-position of a flavone, wherein the polynucleotide is selected from the group consisting of: (a) a polynucleotide composed of the base sequence of SEQ ID NO: 1; (b) a polynucleotide that hybridizes under stringent conditions with a polynucleotide composed of a base sequence complementary to the base sequence of SEQ ID NO: 1; (c) a polynucleotide encoding a protein composed of the amino acid sequence of SEQ ID NO: 2; and, (d) a polynucleotide encoding a protein composed of an amino acid sequence in which one or a plurality of amino acids have been deleted, substituted, inserted and/or added in the amino acid sequence of SEQ ID NO:2.

First claim

The invention claimed is:

1. A recombinant expression vector comprising a polynucleotide operably linked to a heterologous promoter, wherein the polynucleotide is selected from the group consisting of the following (a) to (d):
(a) a polynucleotide comprising the nucleotide sequence of SEQ ID NO: 1;
(b) a polynucleotide encoding a protein comprising the amino acid sequence of SEQ ID NO: 2;
(c) a polynucleotide encoding a protein having one to five amino acids deleted, substituted, or inserted in the amino acid sequence of SEQ ID NO: 2, and having an activity of transferring a sugar group to the hydroxyl group at the 4′-position of a flavone; and,
(d) a polynucleotide encoding a protein having an amino acid sequence identity of 95% or more with SEQ ID NO: 2, and having an activity of transferring a sugar group to the hydroxyl group at the 4′-position of a flavone.

2. A non-human host transformed by the recombinant expression vector of claim 1 .

3. A plant transformed by the recombinant expression vector of claim 1 , a progeny thereof, a portion of the transformed plant or the progeny, or a tissue of the transformed plant or the progeny, wherein each of the plant, the progeny, the portion, and the tissue comprises the recombinant vector.

4. A portion of the plant of claim 3 , wherein the portion is a cut flower.

5. A processed cut flower obtained from the cut flower of claim 4 .

6. A method for producing a protein having an activity of transferring a sugar group to the hydroxyl group at the 4′-position of a flavone, comprising the following steps:
culturing or growing the non-human host of claim 2 , and
harvesting, from the non-human host, the protein having the activity of transferring a sugar group to the hydroxyl group at the 4′-position of a flavone.

7. A method for producing a flavone in which a sugar group has been added to the hydroxyl group at the 4′-position thereof, comprising the following steps:
culturing or growing the non-human host of claim 2 , and
harvesting, from the non-human host, the flavone in which a sugar group has been added to the hydroxyl group at the 4′-position thereof.

8. The recombinant expression vector of claim 1 , wherein the heterologous promoter is selected from the group consisting of a bacterial promoter, a yeast promoter, a filamentous fungi promoter, and a promoter for animal cell hosts.

9. The recombinant expression vector of claim 1 , wherein the heterologous promoter constitutively expresses a gene in plant cells.

10. The recombinant expression vector of claim 9 , wherein the heterologous promoter is selected from the group consisting of cauliflower mosaic virus 35S RNA promoter, rd29A gene promoter, rbcS promoter, and mac-1 promoter.

11. The recombinant expression vector of claim 1 , wherein the heterologous promoter specifically expresses a gene in a plant tissue.

Assignees

• Suntory Holdings Ltd

Inventors

• Tanaka Yoshikazu
• Okitsu Naoko
• Matsui Keisuke

Classifications

• A61P35/00

  Antineoplastic agents · CPC title

• C12N15/825

  involving pigment biosynthesis · CPC title

• A61K8/602

  Glycosides, e.g. rutin · CPC title

• C12P19/18

  produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins · CPC title

• C12N15/8257 Primary

  for the production of primary gene products, e.g. pharmaceutical products, interferon · CPC title