Method of vitally supporting microalgae in a flexible bioreactor

Patent metadata

Field	Value
Publication number	US-10059918-B2
Application number	US-201615142964-A
Country	US
Kind code	B2
Filing date	Apr 29, 2016
Priority date	Mar 31, 2015
Publication date	Aug 28, 2018
Grant date	Aug 28, 2018

Abstract

Some embodiments include a method. The method can include: inoculating a bioreactor with one or more first microorganisms and a first fluidic support medium, the bioreactor having one or more bioreactor walls at least partially enclosing a bioreactor cavity, being configured to be at least one of folded up or rolled up, and being sterile when the bioreactor is inoculated with the one or more first microorganisms, the one or more bioreactor walls having at least one bioreactor wall material, and the at least one bioreactor wall material being flexible and at least partially transparent; and vitally supporting the one or more first microorganisms with the bioreactor, a supply of light, and a supply of organic carbon. Other embodiments of related systems and methods are also disclosed.

First claim

What is claimed is:

1. A method comprising:
inoculating a bioreactor with one or more first microorganisms and a first fluidic support medium, wherein the bioreactor comprises one or more bioreactor walls and one or more bioreactor fittings, the one or more bioreactor walls together with the one or more bioreactor fittings fully enclose a bioreactor cavity, the bioreactor is configured to be gathered up, the bioreactor is sterile when the bioreactor is inoculated with the one or more first microorganisms, the one or more bioreactor walls comprise at least one bioreactor wall material, the at least one bioreactor wall material is flexible and at least partially transparent;
vitally supporting the one or more first microorganisms with the bioreactor, a supply of light, and a supply of organic carbon, such that when the one or more first microorganism are taxonomically classified in the taxonomic family Chlorellaceae, at least one of the average density of the one or more first microorganisms is greater than or equal to approximately 36 grams per liter or the average maximum production rate of the one or more first microorganisms is greater than or equal to approximately 9 grams per liter per day;
after vitally supporting the one or more first microorganisms with the bioreactor, applying water to the bioreactor cavity and gathering up the assembled bioreactor to include the one or more bioreactor walls and the one or more bioreactor fittings, wherein gathering up the assembled bioreactor comprises at least one of folding up the bioreactor or rolling up the bioreactor;
autoclaving the bioreactor while the bioreactor is assembled, gathered up, and containing water;
after autoclaving the bioreactor, inoculating the bioreactor with one or more second microorganisms, the bioreactor being sterile when the bioreactor is inoculated with the one or more second microorganisms; and
after inoculating the bioreactor with the one or more second microorganisms, vitally supporting the one or more second microorganisms with the bioreactor.

2. The method of claim 1 wherein:
the one or more bioreactor fittings comprise at least one gas delivery fitting; and
the bioreactor further comprises:
one or more gas delivery devices located within the bioreactor cavity, the one or more gas delivery devices being operable to inject gas into the bioreactor cavity to mix the one or more microorganisms; and
one or more flexible tubes located within the bioreactor cavity, the one or more flexible tubes comprising at least one gas delivery tube coupling the one or more gas delivery devices to the at least one gas delivery fitting.

3. The method of claim 2 wherein:
autoclaving the bioreactor while the bioreactor is assembled to include the one or more bioreactor walls and the one or more bioreactor fittings further comprises autoclaving the bioreactor while the bioreactor is assembled to include the one or more bioreactor walls, the one or more bioreactor fittings, the one or more flexible tubes, and the one or more gas delivery devices.

4. The method of claim 2 wherein:
when the bioreactor is assembled to include the one or more bioreactor walls, the one or more bioreactor fittings, the one or more flexible tubes, and the one or more gas delivery devices, the bioreactor further comprises a greatest physical dimension; and
the bioreactor, as assembled, is configured to be gathered up, wherein the gathered up assembled bioreactor comprises at least one of folded up or rolled up such that the greatest physical dimension is reducible by at least approximately 75%.

5. The method of claim 2 wherein:
vitally supporting the one or more first microorganism with the bioreactor comprises:
placing a parameter sensing means at a bioreactor fitting of the one or more bioreactor fittings; and
monitoring a cavity environment condition at the bioreactor cavity with the parameter sensing means.

6. The method of claim 1 further comprising:
mechanically supporting the bioreactor with a support structure comprising a first frame and a second frame together being operable to mechanically support the bioreactor in interposition between the first frame and the second frame, wherein the first frame comprises two or more first frame rails comprising a hollow first frame rail conduit and each first frame rail is approximately planar with the other first frame rails and spaced along the plane relative to the other first frame rails, and the first frame rails are configured to form a floor gap disposed at a base of the first frame between a floor and a lowest first frame rail; and
conveying a temperature maintenance fluid through the hollow first frame rail conduit of the two or more first frame rails to maintain a set point temperature of the bioreactor through an exchange of thermal energy between the hollow first frame rail conduit and the bioreactor, wherein the hollow first frame rail conduit simultaneously provides mechanical support to the bioreactor and vitally supports the one or more first microorganism in the bioreactor by maintaining a set point temperature of the bioreactor.

7. The method of claim 6 , further comprising:
relieving stress at a base end of the bioreactor disposed closest to the floor by expanding the flexible material of at least one bioreactor wall at the base end from between the first frame and second frame into the floor gap.

8. The method of claim 1 wherein:
vitally supporting the one or more first microorganisms with the bioreactor comprises:
mixing the one or more first microorganisms within the first fluidic support medium by injecting gas into the first fluidic support medium;

and
the gas comprises gas bubbles comprising a diameter greater than or equal to approximately 40 micrometers and less than or equal to approximately 2 millimeters.

9. The method of claim 1 wherein:
vitally supporting the one or more first microorganisms with the bioreactor comprises:
mixing the one or more first microorganisms within the first fluidic support medium by injecting gas into the first fluidic support medium at a volumetric flow rate of greater than or equal to approximately 10 liters per minute and less than or equal to approximately 60 liters per minute.

10. A method comprising:
inoculating a bioreactor with one or more first microorganisms and a first fluidic support medium, wherein the bioreactor comprises one or more bioreactor walls and one or more bioreactor fittings, the one or more bioreactor walls together with the one or more bioreactor fittings fully enclose a bioreactor cavity, the bioreactor is configured to be gathered up, the bioreactor is sterile when the bioreactor is inoculated with the one or more first microorganisms, the one or more bioreactor walls comprise at least one bioreactor wall material, the at least one bioreactor wall material is flexible and at least partially transparent;
vitally supporting the one or more first microorganisms with the bioreactor, a supply of light, and a supply of organic carbon, such that when the one or more first microorganism are taxonomically classified in the taxonomic family Chlorellaceae, at least one of the average density of the one or more first microorganisms is greater than or equal to approximately 36 grams per liter or the average maximum production rate of the one or more first microorganisms is greater than or equal to approximately 9 grams per liter per day;
mechanically supporting the bioreactor with a support structure comprising a first frame and a second frame together being operable to mechanically support the bioreactor in interposition between the first frame and the second frame, wherein the first frame comprises two or more first frame rails

Assignees

• Heliae Dev Llc
• Heliae Dev Llc

Inventors

• Cizek Luke
• Oelschlager Mason
• Hayden Justin
• Olaizola Miguel
• Mccarty Mason
• Mazur Richard

Classifications

• C12M37/00

  Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination (C12M23/38 takes precedence; filtration in general and filters per se B01D24/00-B01D41/00; autoclaves B01J3/04; treatment of microorganisms with electrical or wave energy C12N13/00) · CPC title

• C12M41/22

  in contact with the bioreactor walls · CPC title

• C12N1/12 Primary

  Unicellular algae; Culture media therefor (as new plants A01H13/00) · CPC title

• C12M29/04

  Filters; Permeable or porous membranes or plates, e.g. dialysis · CPC title

• C12M41/40

  of pressure · CPC title