Composition for enzymatic debridement

Patent metadata

Field	Value
Publication number	US-10058596-B2
Application number	US-201213719176-A
Country	US
Kind code	B2
Filing date	Dec 18, 2012
Priority date	Dec 20, 2011
Publication date	Aug 28, 2018
Grant date	Aug 28, 2018

Abstract

Methods and compositions for the enzymatic debridement of wounds are provided. Methods for preparing enzymatic debridement compositions are also provided. In one embodiment, an enzymatic debridement composition prepared by dissolving crude bromelain in a composition comprising a weak acid, and filtering and/or dialyzing the dissolved crude bromelain to obtain the enzymatic debridement composition is provided.

First claim

The invention claimed is:

1. A method of preparing an enzymatic debridement composition wherein the method consists of:
(a) dissolving crude bromelain in a composition comprising a weak acid;
(b) filtering and/or dialyzing the dissolved crude bromelain to obtain the enzymatic debridement composition; and
(c) optionally lyophilizing the enzymatic debridement composition.

2. The method of claim 1 , wherein the method consists of:
(a) dissolving crude bromelain in a composition comprising a weak acid;
(b) filtering the dissolved crude bromelain to obtain a filtered bromelain composition;
(c) dialyzing the filtered bromelain composition to obtain the enzymatic debridement composition; and
(d) optionally lyophilizing the enzymatic debridement composition.

3. The method of claim 1 , wherein the weak acid is selected from the group consisting of acetic acid, trichloroacetic acid, oxalic acid, formic acid, benzoic acid, and citric acid.

4. The method of claim 1 , wherein the enzymatic debridement composition is in the form of liquid or powder.

5. A method of preparing an enzymatic debridement composition wherein the method consists of:
(a) dissolving crude bromelain in a composition comprising a weak acid;
(b) filtering and/or dialyzing the dissolved crude bromelain to obtain the enzymatic debridement composition;
(c) optionally lyophilizing the enzymatic debridement composition to obtain a lyophilized enzymatic debridement composition; and
(d) formulating the lyophilized enzymatic debridement composition into a liquid, a powder, a film, a gel, a foam, or a spray.

6. The method of claim 5 , wherein the gel comprises 10% by weight of the lyophilized enzymatic debridement composition.

7. The method of claim 3 , wherein the crude bromelain is dissolved in a composition comprising 0.3M acetic acid.

8. The method of claim 1 , wherein the dissolved crude bromelain is filtered through a 0.2μm pore filter.

9. The method of claim 1 , wherein the filtered bromelain is dialyzed through a 12,400 molecular weight cut-off membrane.

10. A method of preparing an enzymatic debridement composition wherein the method consists of:
(a) dissolving crude bromelain in a composition comprising a weak acid;
(b) filtering and/or dialyzing the dissolved crude bromelain to obtain the enzymatic debridement composition;
(c) optionally lyophilizing the enzymatic debridement composition to obtain a lyophilized enzymatic debridement composition; and
(d) applying the enzymatic debridement composition or the lyophilized enzymatic debridement composition on a wound dressing.

11. A method for debridement of devitalized tissue from a subject consisting of:
(a) dissolving crude bromelain in a composition comprising a weak acid;
(b) filtering and/or dialyzing the dissolved crude bromelain to obtain the enzymatic debridement composition;
(c) optionally lyophilizing the enzymatic debridement composition to obtain a lyophilized enzymatic debridement composition;
(d) contacting the devitalized tissue with the enzymatic debridement composition or the lyophilized enzymatic debridement composition to dissolve the devitalized tissue; and
(e) removing the dissolved devitalized tissue.

12. The method of claim 11 , wherein the method consists of:
(a) dissolving crude bromelain in a composition comprising a weak acid;
(b) filtering the dissolved crude bromelain to obtain a filtered bromelain composition;
(c) dialyzing the filtered bromelain composition to obtain the enzymatic debridement composition;
(d) optionally lyophilizing the enzymatic debridement composition to obtain a lyophilized enzymatic debridement composition;
(e) contacting the devitalized tissue with the enzymatic debridement composition or the lyophilized enzymatic debridement composition to dissolve the devitalized tissue; and
(f) removing the dissolved devitalized tissue.

13. The method of claim 11 , wherein the enzymatic debridement composition is in contact with the devitalized tissue for 1 to 48 hours before the dissolved devitalized tissue is removed.

14. The method of claim 11 , wherein contacting the devitalized tissue is by coating the devitalized tissue with the enzymatic debridement composition or by injecting the enzymatic debridement composition into the devitalized tissue.

Assignees

• Kci Licensing Inc

Inventors

• Hanson Douglas Philip

Classifications

• A61K9/06

  Ointments; Bases therefor; {Other semi-solid forms, e.g. creams, sticks, gels (composition of ointments, creams or gels A61K47/00)} · CPC title

• A61K38/4873

  Cysteine endopeptidases (3.4.22), e.g. stem bromelain, papain, ficin, cathepsin H · CPC title

• A61K9/0014 Primary

  Skin, i.e. galenical aspects of topical compositions (non-active ingredients are additionally classified in A61K47/00; A61K9/0009, A61K9/0021, A61K9/7015, A61K9/7023 take precedence; cosmetic preparations A61K8/00, A61Q; preparations for wound dressings or bandages A61L26/00) · CPC title

• A61K38/43 Primary

  Enzymes; Proenzymes; Derivatives thereof · CPC title