Microscope

Patent metadata

Field	Value
Publication number	US-10054780-B2
Application number	US-201414249921-A
Country	US
Kind code	B2
Filing date	Apr 10, 2014
Priority date	Dec 20, 2007
Publication date	Aug 21, 2018
Grant date	Aug 21, 2018

Abstract

A microscope including an imaging objective for imaging a sample on a detector and means for illuminating the sample with a light sheet in the focus plane of the imaging objective. The illumination means includes an illumination source which emits coherent light, and Bessel optics which generate at least two plane waves from the light beam and give propagation directions for the plane waves. The propagation direction of each of the plane waves encloses an acute angle with the focus plane in each instance, the magnitude of the acute angle being identical for each of the plane waves, so that the plane waves undergo constructive interference in the focus plane so that a light sheet is generated. Similarly, the illumination means can also include an optical element by which a rotationally symmetric Bessel beam is generated from the light beam for dynamic generation of a light sheet.

First claim

The invention claimed is:

1. A microscope comprising:
an imaging objective for imaging a sample on a detector and having a focus plane where the sample is located; and
means for illuminating the sample with a light sheet in the focus plane of the imaging objective;
wherein the illumination means comprise:
an illumination source which emits coherent light;
an optical element by which a rotationally symmetric Bessel beam is generated;
a first lens;
a second lens; and
scanning means by which the Bessel beam scans the sample in the focus plane of the imaging objective

wherein the optical element, the first lens, and the second lens are designed and arranged corresponding to a 4f geometry with a Fourier plane between the first and second lenses so that the Bessel beam is imaged in the focus plane of the imaging objective,
wherein the illumination means is configured to generate constructive interference by the Bessel beam at the focus plane to produce the light sheet, and
wherein an optical axis of the imaging objective extends perpendicular to an illumination direction.

2. The microscope according to claim 1 , wherein the second lens generates two plane waves and gives propagation directions to the two plane waves that are at acute angles to the focus plane of the imaging objective such that the two plane waves constructively interfere with each other at the focus plane to produce the light sheet.

3. The microscope according to claim 2 , wherein the two plane waves run toward the focus plane from a same side of the imaging objective.

4. The microscope according to claim 1 , wherein, at the focus plane, the optical axis of the imaging objective extends perpendicular to the illumination direction.

Assignees

• Zeiss Carl Microscopy Gmbh

Inventors

• Lippert Helmut
• Wolleschensky Ralf
• Rohrbach Alexander
• Fahrbach Florian

Classifications

• G02B26/0816

  by means of one or more reflecting elements · CPC title

• G02B21/10

  affording dark-field illumination (G02B21/14 {and G02B21/125} take precedence) · CPC title

• G02B21/06 Primary

  Means for illuminating specimens · CPC title

• G02B21/16

  adapted for ultraviolet illumination {; Fluorescence microscopes (G02B21/0076 takes precedence)} · CPC title

• G02B27/0911

  Anamorphotic systems · CPC title