Protein having glycoalkaloid biosynthetic enzyme activity and gene encoding the same

Patent metadata

Field	Value
Publication number	US-10053701-B2
Application number	US-201214358568-A
Country	US
Kind code	B2
Filing date	Nov 19, 2012
Priority date	Nov 17, 2011
Publication date	Aug 21, 2018
Grant date	Aug 21, 2018

Abstract

Provided is DNA of biosynthetic enzyme of glycoalkaloid of solanaceous plant (Solanaceae) such as potato. Provided are a protein having activity on a biosynthetic enzyme of glycoalkaloid of solanaceous plant such as potato, and a method of creating and assaying a novel organism using a gene encoding the protein.

First claim

The invention claimed is:

1. A method of producing a plant belonging to the family Solanaceae having reduced accumulation of glycoalkaloids with respect to an existing variety, said method comprising suppressing the expression of a gene consisting of a DNA selected from the group consisting of (a)-(c) by mutagenesis, RNA interference, or by modifying the plant genome itself:
(a) a DNA consisting of the nucleotide sequence of SEQ ID NO: 2 or SEQ ID NO: 4;
(b) a DNA encoding a protein consisting of the amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 3; and
(c) a DNA encoding a protein having at least 95% identity with the amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 3 and having aminotransferase activity;
thereby producing the Solanaceae plant with reduced accumulation of glycoalkaloids.

Assignees

• Kirin Holdings Kk

Inventors

• Umemoto Naoyuki

Classifications

• C12Y206/01019

  4-Aminobutyrate—2-oxoglutarate transaminase (2.6.1.19) · CPC title

• C07K14/415

  from plants · CPC title

• A01H5/04

  Stems · CPC title

• C12Y206/01

  Transaminases (2.6.1) · CPC title

• C12Q2600/156

  Polymorphic or mutational markers · CPC title