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Recombinant microorganisms of Escherichia with L-threonine productivity and method of producing L-threonine using the same
US10053698B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10053698-B2 |
| Application number | US-201415112992-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 25, 2014 |
| Priority date | Feb 13, 2013 |
| Publication date | Aug 21, 2018 |
| Grant date | Aug 21, 2018 |
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- Title
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Abstract
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The present invention relates to an E. coli mutant strain having enhanced L-threonine productivity, which is obtained by introducing the permease of Corynebacterium origin, and to method of producing L-threonine using the E. coli mutant strain.
First claim
Opening claim text (preview).
The invention claimed is: 1. A recombinant microorganism of the genus Escherichia having an enhanced rate of producing L-threonine as compared to the parent strain, wherein the recombinant microorganism is obtained by transformation so as to contain permease of Corynebacterium origin having SEQ ID NO: 1 or SEQ ID NO: 2. 2. The recombinant microorganism of the genus Escherichia according to claim 1 , wherein the recombinant microorganism is Escherichia coli. 3. The recombinant microorganism of the genus Escherichia according to claim 1 , wherein the recombinant microorganism is obtained by transformation so as to contain both the Corynebacterium permeases of SEQ ID NO: 1 and SEQ ID NO: 2. 4. A method for producing L-threonine, comprising the steps of: inoculating and culturing the microorganism of claim 1 ; and separating L-amino acid from the culture. 5. A method for producing L-threonine, comprising the steps of: inoculating and culturing the microorganism of claim 2 ; and separating L-amino acid from the culture. 6. A method for producing L-threonine, comprising the steps of: inoculating and culturing the microorganism of claim 3 ; and separating L-amino acid from the culture.
Assignees
Inventors
Classifications
- C12N1/20Primary
Bacteria; Culture media therefor · CPC title
Lysine; Diaminopimelic acid; Threonine; Valine · CPC title
- C12N15/70Primary
Vectors or expression systems specially adapted for E. coli · CPC title
- C07K14/34Primary
from Corynebacterium (G) · CPC title
DNA sequences coding for fusion proteins · CPC title
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Frequently asked questions
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- What does patent US10053698B2 cover?
- The present invention relates to an E. coli mutant strain having enhanced L-threonine productivity, which is obtained by introducing the permease of Corynebacterium origin, and to method of producing L-threonine using the E. coli mutant strain.
- Who is the assignee on this patent?
- Cj Cheiljedang Corp
- What technology area does this patent fall under?
- Primary CPC classification C12N1/20. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Aug 21 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).