Recombinant expression vector applicable to rapid screening for recombinant strain and application
US-12037632-B2 · Jul 16, 2024 · US
Cellulase gene
US10053679B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10053679-B2 |
| Application number | US-201013638538-A |
| Country | US |
| Kind code | B2 |
| Filing date | Mar 31, 2010 |
| Priority date | Mar 31, 2010 |
| Publication date | Aug 21, 2018 |
| Grant date | Aug 21, 2018 |
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- Title
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Abstract
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An object is to identify endoglucanase and β-glucosidase genes by isolating genomic DNA containing cellulase genes, which are classified into endoglucanases or β-glucosidases, from Acremonium cellulolyticus , and sequencing the nucleotide sequences thereof. The inventors intensively compared the amino acid sequences of known endoglucanases and β-glucosidases with each other to find conserved region of amino acid sequences in Acremonium cellulolyticus , and various primers were designed based on the information. PCR was carried out using the various primers thus designed and genomic DNA or cDNA as a template. As a result, gene fragments of endoglucanases and β-glucosidases were obtained. Primers were designed based on the gene fragments, and PCR was carried out to amplify nine genes of endoglucanases and β-glucosidases. The nucleotide sequences thereof were sequenced, and the present invention was completed.
First claim
Opening claim text (preview).
The invention claimed is: 1. A composition comprising a protein and an effective amount of an added preservative, wherein said protein consists of the amino acid sequence of residues 1-301 of SEQ ID NO: 14. 2. A protein having endoglucanase activity, wherein said protein consists of the amino acid sequence of residues 1-301 of SEQ ID NO: 14 with a single substitution.
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Classifications
Treatment of cellulose-containing material with microorganisms or enzymes · CPC title
produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title
Beta-glucosidase (3.2.1.21) · CPC title
Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase · CPC title
Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source · CPC title
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- What does patent US10053679B2 cover?
- An object is to identify endoglucanase and β-glucosidase genes by isolating genomic DNA containing cellulase genes, which are classified into endoglucanases or β-glucosidases, from Acremonium cellulolyticus , and sequencing the nucleotide sequences thereof. The inventors intensively compared the amino acid sequences of known endoglucanases and β-glucosidases with each other to find conserved r…
- Who is the assignee on this patent?
- Yokoyama Fumikazu, Meiji Seika Pharma Co Ltd
- What technology area does this patent fall under?
- Primary CPC classification C12N9/2437. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Aug 21 2018 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).