Microbubble tether for diagnostic and therapeutic applications

Patent metadata

Field	Value
Publication number	US-10052394-B2
Application number	US-201414550689-A
Country	US
Kind code	B2
Filing date	Nov 21, 2014
Priority date	Nov 21, 2014
Publication date	Aug 21, 2018
Grant date	Aug 21, 2018

Abstract

The present disclosure relates to a composition of albumin microbubbles to which are bound one or more moieties that exhibit a binding preference for the albumin microbubbles relative to free, native HSA. Production of the albumin microbubble composition and use of the albumin microbubble composition in ultrasound mediated delivery of therapeutic or diagnostic agents is also discussed.

First claim

The invention claimed is:

1. A composition, comprising:
a microbubble comprising an albumin shell;
a therapeutic agent or a diagnostic agent; and
a moiety bound to the microbubble and to the therapeutic agent or the diagnostic agent, wherein the moiety hydrophobically binds preferentially to the microbubble relative to native albumin, and wherein the moeity is a cyanine 5 derivative comprising polyethyleneglycol units.

2. The composition of claim 1 , wherein the therapeutic agent or the diagnostic agent comprises a small molecule drug, an oligonucleotide, or a large molecule.

3. The composition of claim 1 , wherein the albumin shell comprises partially denatured, partially cross-linked human serum albumin.

4. The composition of claim 1 , wherein the microbubble comprises a perfluoropropane core.

5. The composition of claim 1 , wherein the microbubble has a diameter between about 1 μm to about 10 μm.

6. A composition, comprising:
a microbubble comprising an albumin shell;
a therapeutic agent or a diagnostic agent; and
a moiety bound to the microbubble and to the therapeutic agent or the diagnostic agent, wherein the moiety hydrophobically binds preferentially to the microbubble relative to native albumin, wherein the moeity is a cyanine 5 derivative, and wherein the cyanine 5 derivative comprises the following structure:

7. The composition of claim 1 , wherein the cyanine 5 derivative comprises the following structures:

wherein n is 10, or

wherein n is 450.

Assignees

• Gen Electric

Inventors

• Butts Matthew David
• Castle Jason William
• Torres Andrew Soliz
• Roberts Jeannette Christine
• Kandapallil Binil Itty Ipe

Classifications

• A61K9/0009 Primary

  involving or responsive to electricity, magnetism or acoustic waves; Galenical aspects of sonophoresis, iontophoresis, electroporation or electroosmosis · CPC title

• A61K49/223 Primary

  Microbubbles, hollow microspheres, free gas bubbles, gas microspheres · CPC title

• A61K9/5052

  Proteins, e.g. albumin · CPC title