Recombinant microorganism for improved production of fine chemicals

Patent metadata

Field	Value
Publication number	US-10047364-B2
Application number	US-201415024464-A
Country	US
Kind code	B2
Filing date	Sep 11, 2014
Priority date	Sep 25, 2013
Publication date	Aug 14, 2018
Grant date	Aug 14, 2018

Abstract

The present invention relates to a recombinant nucleic acid molecule, a recombinant microorganism, to a method for producing alanine and to the use of the recombinant nucleic acid molecule or the recombinant microorganism for the fermentative production of alanine.

First claim

We claim:

1. A recombinant nucleic acid molecule encoding an alanine transporter and having a sequence selected from the group of
(I) a nucleic acid molecule comprising the sequence of SEQ ID NO: 1, wherein the codon corresponding to position 13-15 of SEQ ID NO: 1 does not encode amino acid glutamine and is not a stop codon,
(II) a nucleic acid molecule encoding a polypeptide having the sequence of SEQ ID NO: 2, wherein the amino acid of the polypeptide corresponding to position 5 of SEQ ID NO: 2 is not glutamine, and
(III) a nucleic acid molecule encoding a polypeptide having at least 95% sequence identity to a polypeptide having the sequence of SEQ ID NO: 2, wherein the amino acid of the polypeptide corresponding to position 5 of SEQ ID NO: 2 is not glutamine.

2. The recombinant nucleic acid molecule of claim 1 having the sequence of SEQ ID NO: 1, wherein the nucleic acid molecule encodes, at a position corresponding to position 5 of SEQ ID NO: 2, the amino acid histidine or another basic amino acid, the amino acid asparagine or another aliphatic amino acid, the amino acid arginine or another aliphatic amino acid, or the amino acid tyrosine or another aromatic amino acid.

3. A recombinant expression construct comprising at least one promoter functional in a microorganism operably linked to the nucleic acid molecule of claim 1 .

4. A recombinant vector comprising the nucleic acid molecule of claim 1 .

5. A recombinant microorganism comprising the nucleic acid of claim 1 .

6. A composition comprising the recombinant microorganism according to claim 5 .

7. A method of producing pyruvate, succinate, aspartate, malate, lactate, valine, leucine and/or alanine comprising culturing the recombinant microorganism according to claim 5 under conditions that allow for the production of pyruvate, succinate, aspartate, malate, lactate, valine, leucine, and/or alanine.

8. A process for fermentative production of pyruvate, succinate, aspartate, malate, lactate, valine, leucine and/or alanine comprising the steps of
I) growing the microorganism according to claim 5 in a fermenter and
II) recovering pyruvate, succinate, aspartate, malate, lactate, valine, leucine and/or alanine from the fermentation broth obtained in I).

Assignees

• Basf Se

Inventors

• Schröder Hartwig
• Hartmann Holger
• Wang Qingzhao
• Ratani Shakir
• Guo Zheyuan
• Pompejus Markus

Classifications

• C12P13/06

  Alanine; Leucine; Isoleucine; Serine; Homoserine · CPC title

• C12N15/70 Primary

  Vectors or expression systems specially adapted for E. coli · CPC title

• C12N9/16

  acting on ester bonds (3.1) · CPC title

• C12N9/0036

  acting on NADH or NADPH (1.6) · CPC title

• C07K14/245 Primary

  Escherichia (G) · CPC title