Soy gene cluster regions and methods of use

Patent metadata

Field	Value
Publication number	US-10045494-B2
Application number	US-201514967716-A
Country	US
Kind code	B2
Filing date	Dec 14, 2015
Priority date	Mar 16, 2012
Publication date	Aug 14, 2018
Grant date	Aug 14, 2018

Abstract

Methods for conveying pathogen resistance into non-resistant soybean germplasm are provided. In some embodiments, the methods include introgressing pathogen resistance into a non-resistant soybean using one or more nucleic acid markers for marker-assisted breeding among soybean lines to be used in a soybean breeding program, wherein the markers are linked to and/or associated with pathogen resistance. Also provided are single nucleotide polymorphisms (SNPs) associated with resistance to pathogens; soybean plants, seeds, and tissue cultures produced by any of the disclosed methods; seed produced by the disclosed soybean plants; and compositions including amplification primer pairs capable of initiating DNA polymerization by a DNA polymerase on soybean nucleic acid templates to generate soybean marker amplicons.

First claim

What is claimed is:

1. A method of producing a soybean progeny plant having improved resistance to Phytophthora , the method comprising the steps of:
a. isolating nucleic acids from a soybean plant selected from a population of soybean plants;
b. detecting in the nucleic acids of (a) at least one allele of one or more marker locus associated with improved resistance to Phytophthora wherein the one or more marker locus is a marker locus localizing within a chromosomal interval on soy chromosome 13 comprising SEQ ID NO: 6 and further having a T corresponding to position 251;
c. selecting a first soybean plant from the population of (a) based on the presence of the allele detected in (b);
d. crossing the first soybean plant of (c) with a second soybean plant wherein said second soybean plant does not comprise the one or more marker locus of (b) associated with improved Phytophthora resistance;
e. collecting seed from the cross of (d); and
f. growing a soybean progeny plant from the seed of (e), wherein said soybean progeny plant comprises in its genome said marker locus associated with improved resistance to Phytophthora , thereby producing a soybean progeny plant with improved resistance to Phytophthora.

2. The method of claim 1 , wherein the allele associated with improved Phytophthora resistance corresponds to a T at position 251 of SEQ ID NO: 6.

3. The method of claim 1 , wherein the detecting comprises detecting at least one allelic form of a polymorphic simple sequence repeat (SSR) or a single nucleotide polymorphism (SNP).

4. The method of claim 1 , wherein the detecting comprises amplifying the marker locus or a portion of the marker locus and detecting the resulting amplified marker amplicon.

Assignees

• Syngenta Participations Ag

Inventors

• Yu Ju-Kyung
• Breitinger Becky Welsh
• Plunkett David
• Shi Ainong
• Dyer Daniel

Classifications

• C12Q1/6895

  for plants, fungi or algae · CPC title

• C12Q2600/172

  Haplotypes · CPC title

• C12N15/8279

  for biotic stress resistance, pathogen resistance, disease resistance · CPC title

• C12Q2600/156

  Polymorphic or mutational markers · CPC title

• C12N15/8282

  for fungal resistance · CPC title