Imaging probe including nanoparticle

Patent metadata

Field	Value
Publication number	US-10041951-B2
Application number	US-201313755613-A
Country	US
Kind code	B2
Filing date	Jan 31, 2013
Priority date	Jan 31, 2012
Publication date	Aug 7, 2018
Grant date	Aug 7, 2018

Abstract

An imaging probe can include a photoluminescent carbon nanostructure configured to emit a wavelength of light detectable through living tissue, and a targeting moiety including a first binding partner configured to interact with a second binding partner.

First claim

What is claimed is:

1. An imaging probe, comprising:
a photoluminescent nanostructure that emits a wavelength of light detectable through living tissue and a targeting moiety;
wherein the photoluminescent nanostructure is complexed with the targeting moiety,
wherein the targeting moiety comprises a first binding partner and at least one viral protein; and
further wherein the photoluminescent nanostructure is a single walled carbon nanotube, the at least one viral protein is an M13 bacteriophage protein, and the first binding partner is selected from the group consisting of an antibody against prostate specific membrane antigen (anti-PSMA antibody), an anti- Staphylococcus aureus antibody, and a secreted protein acidic and rich in cysteine (SPARC)-binding peptide.

2. The imaging probe of claim 1 , wherein the first binding partner binds a second binding partner, wherein the second binding partner is selected from the group consisting of a tissue-type specific molecule and a cell-type specific molecule.

3. The imaging probe of claim 2 , wherein the second binding partner is a protein.

4. The imaging probe of claim 1 , wherein the first binding partner is selected from the group consisting of an anti-PSMA antibody, an anti- Staphylococcus aureus antibody, and a fragment thereof.

5. The imaging probe of claim 1 , wherein the M13 bacteriophage protein is a capsid M13 bacteriophage protein.

6. The imaging probe of claim 2 , wherein the first binding partner binds the second binding partner in vivo.

7. The imaging probe of claim 2 , wherein the first binding partner binds the second binding partner ex vivo.

8. The imaging probe of claim 1 , wherein the imaging probe binds tumors located at various depths in the body.

9. The imaging probe of claim 1 , wherein the imaging probe binds tumors located at depths of 9.7 to 18.2 millimeters below the surface of a tissue containing the tumors.

10. The imaging probe of claim 1 , wherein the imaging probe differentiates between a tumor and a healthy tissue.

11. The imaging probe of claim 1 , wherein the imaging probe has a fluorescence stability of at least 24 hours in vivo.

12. The imaging probe of claim 1 , wherein the imaging probe fluorescence is stable at pH 4.5 to pH 8.5.

13. The imaging probe of claim 1 , wherein the imaging probe is not cytotoxic to an ovarian cell line.

14. The imaging probe of claim 1 , wherein the first binding partner is a SPARC-binding peptide.

15. A method comprising:
(a) applying the imaging probe of claim 1 to a sample;
(b) exposing at least a portion of the sample to an excitation light in order to cause an emission from the imaging probe; and
(c) detecting the emission from the imaging probe.

16. The method of claim 15 , wherein the first binding partner of the imaging probe binds a second binding partner, and wherein the second binding partner is selected from the group consisting of a tissue-type specific molecule and a cell-type specific molecule.

17. The method of claim 16 , wherein the second binding partner is a protein.

18. The method of claim 15 , wherein the first binding partner of the imaging probe is selected from the group consisting of an anti-PSMA antibody, an anti- Staphylococcus aureus antibody, and a fragment thereof.

19. The method of claim 15 , wherein the M13 bacteriophage protein of the imaging probe is a capsid M13 bacteriophage protein.

20. The method of claim 15 , wherein the detecting step comprises detecting the emission of the imaging probe at a wavelength between 950-1400 nm.

21. The method of claim 15 , wherein the applying step comprises administering the imaging probe to a subject that is a mammal.

22. The method of claim 21 , wherein the method is performed in vivo.

23. The method of claim 15 , wherein the method is performed ex vivo.

24. The method of claim 15 , wherein the imaging probe binds tumors located at various depths in the body.

25. The method of claim 15 , wherein the imaging probe binds tumors located at depths of 9.7 to 18.2 millimeters below the surface of a tissue containing the tumors.

26. The method of claim 15 , wherein the method is for image-guided surgery, and wherein detecting the emission from the imaging probe reveals tumors for surgical procedure.

27. The method of claim 26 , wherein the surgical procedure is cytoreductive surgery.

28. The method of claim 26 , wherein the surgical procedure is an ovarian cancer surgery.

29. The method of claim 15 , wherein the imaging probe differentiates between a tumor and a healthy tissue.

30. The method of claim 15 , wherein the imaging probe fluorescence is stable for at least 24 hours in vivo.

31. The method of claim 15 , wherein the imaging probe fluorescence is stable at pH 4.5 to pH 8.5.

32. The method of claim 15 , wherein the imaging probe is not cytotoxic to an ovarian cell line.

33. The method of claim 15 , wherein the first binding partner is a SPARC-binding peptide.

Assignees

• Massachusetts Inst Technology

Inventors

• Yi Hyunjung
• Ghosh Debadyuti
• Qi Jifa
• Belcher Angela M
• Strano Michael S
• Bardhan Neelkanth M

Classifications

• A61K49/0095

  Nanotubes · CPC title

• Y10S977/75

  Single-walled · CPC title

• A61K49/0017 Primary

  Fluorescence in vivo · CPC title

• G01N33/582 Primary

  with fluorescent label · CPC title

• A61K49/0056

  Peptides, proteins, polyamino acids · CPC title