Microfluidic device for separating liquid from the same liquid containing deformable particles without external sources of energy

Patent metadata

Field	Value
Publication number	US-10041931-B2
Application number	US-201615154937-A
Country	US
Kind code	B2
Filing date	May 13, 2016
Priority date	Nov 15, 2013
Publication date	Aug 7, 2018
Grant date	Aug 7, 2018

Abstract

A microfluidic device for separating liquid from the same liquid containing deformable particles without external sources of energy. The device includes: at least one transport channel (1) using various fluid-hydrodynamic principles in order to increase the amount of separated/obtained fluid; and at least one separating area (2) which diverts part of the fluid circulating through the transport channel towards at least one collection channel (3) or reaction chamber. The separating area (2) can include at least one array of pillars, the configuration of which maximizes the amount of fluid extracted at once. The depth and width of the collection channel (3) are adaptable according to the requirements of the test to be implemented. A filter is provided for separating plasma from a drop of blood in a highly efficient manner, which can be used in point-of-care testing systems.

First claim

The invention claimed is:

1. A self-driven microfluidic device for separating particles from a liquid in which the particles are suspended and particularly for blood plasma separation, comprising:
a) a transport channel ( 1 ) divided in three sections: a first channel section ( 1 a ) and a second channel section ( 1 e ) having a same width w 1 and interconnected by an intermediate channel section ( 1 c ) having a width w 2 , where w 2 <w 1 , the first and second channel sections ( 1 a ), ( 1 e ) being connected to said intermediate section ( 1 c ) through a corresponding constriction ( 1 b ) ( 1 d ) having inner walls, said transport channel being configured to transport the liquid from an inlet port ( 11 ) to an outlet port ( 12 ) and avoid clogging due to an increased flow in the corresponding constriction;
b) a separating area ( 2 ), symmetrically located on both sides of the transport channel ( 1 ) and lying completely or partially over the intermediate channel section ( 1 c ), the separating area ( 2 ) being configured to act as a filter; and
c) a collection channel ( 3 ) including an outlet opening ( 31 ) or a reaction chamber, the collection channel ( 3 ) being covered by said separating area ( 2 ),

wherein the microfluidic device ( 100 ) is machined in two parts, a first part ( 101 ) containing the separating area, the separating area having a depth (H 2 ), and a second part ( 102 ) containing the transport channel ( 1 ) and the collection channel ( 3 ), the transport channel ( 1 ) and the collection channel ( 3 ) each having a depth (H 1 ) adapted according to a test/analysis to be performed wherein said depth (H 2 ) being smaller than said depth (H 1 ), and
wherein both said first part ( 101 ) and second part ( 102 ) include a hydrophilic material or are treated to achieve a hydrophilic behavior in a hydrophobic material.

2. The microfluidic device for separation of claim 1 , further comprising at least one micropump, which includes parallel channels ( 41 ) or a collection of micropillars ( 51 ), connected to the end of the second channel section ( 1 e ) to promote flow.

3. The microfluidic device of claim 1 , wherein the transport channel ( 1 ) is curved and the separating area ( 2 ) covers the whole intermediate channel section ( 1 c ).

4. The microfluidic device of claim 2 , wherein the transport channel ( 1 ) is curved and the separating area ( 2 ) covers the whole intermediate channel section ( 1 c ).

5. The microfluidic device of claim 1 , wherein the transport channel 1 is curved and the separating area ( 2 ) covers only a part of the intermediate channel section ( 1 c ).

6. The microfluidic device of claim 2 , wherein the transport channel 1 is curved and the separating area ( 2 ) covers only a part of the intermediate channel section ( 1 c ).

7. The microfluidic device of claim 1 , wherein at least two electrodes ( 4 ) and ( 5 ) are placed at the inlet and outlet of the transport channel ( 1 ) to apply alternating electrophoretic forces on the particles.

8. The microfluidic device of claim 2 , wherein at least two electrodes ( 4 ) and ( 5 ) are placed at the inlet and outlet of the transport channel ( 1 ) to apply alternating electrophoretic forces on the particles.

9. The microfluidic device of claim 1 wherein the separating area includes an arrangement of micropillars ( 2 ).

10. The microfluidic device of claim 2 wherein the separating area includes an arrangement of micropillars ( 2 ).

Assignees

• Univ Catalunya Politecnica

Inventors

• Casals Terré Jasmina
• Madadi Hojjat

Classifications

• B01L3/502753

  characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation · CPC title

• G01N33/491 Primary

  by separating the blood components (G01N15/05 takes precedence) · CPC title

• G01N33/49 Primary

  Blood {(chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80)} · CPC title

• B01L2400/086

  using baffles or other fixed flow obstructions · CPC title

• B01L3/502746

  characterised by the means for controlling flow resistance, e.g. flow controllers, baffles or throttle valves · CPC title