Biopolymer optofluidic device and method of manufacturing the same

Patent metadata

Field	Value
Publication number	US-10040834-B2
Application number	US-201414154134-A
Country	US
Kind code	B2
Filing date	Jan 13, 2014
Priority date	Nov 3, 2006
Publication date	Aug 7, 2018
Grant date	Aug 7, 2018

Abstract

A method of manufacturing a biopolymer optofluidic device including providing a biopolymer, processing the biopolymer to yield a biopolymer matrix solution, providing a substrate, casting the biopolymer matrix solution on the substrate, embedding a channel mold in the biopolymer matrix solution, drying the biopolymer matrix solution to solidify biopolymer optofluidic device, and extracting the embedded channel mold to provide a fluidic channel in the solidified biopolymer optofluidic device. In accordance with another aspect, an optofluidic device is provided that is made of a biopolymer and that has a channel therein for conveying fluid.

First claim

What is claimed is:

1. A device made of a solidified silk fibroin matrix, comprising:
at least one fluidic channel therein;
an embedded organic material; and
at least one optical feature,
wherein the silk fibroin is from at least one of silkworm silk, spider silk, genetically engineered silk, synthetic silk, or combinations thereof,
wherein the device is characterized in that when a fluid flows through the at least one fluidic channel, it interacts with the embedded organic material to alter at least one local optical property of the solidified silk fibroin matrix such that the device is biologically and optically active, and
wherein the at least one local optical property is selected from the group consisting of diffractive property, refractive property, transmissive property, and combinations thereof.

2. The device of claim 1 , wherein the at least one optical feature is at least one of a lens, a microlens array, an optical grating, a pattern generator, and a beam reshaper.

3. The device of claim 1 , wherein a surface of said device comprises features formed in a pattern thereon.

4. The device of claim 1 , wherein said solidified silk fibroin matrix further comprises a biopolymer selected from the group consisting of: chitosan, collagen, gelatin, agarose, chitin, polyhydroxyalkanoates, pullan, starch, amylose, amylopectin, cellulose, hyaluronic acid, and combinations thereof.

5. The device of claim 1 , wherein said organic material is selected from the group consisting of red blood cells, horseradish peroxidase, phenolsulfonphthalein, and combinations thereof.

6. The device of claim 1 , wherein said organic material is selected from the group consisting of a nucleic acid, a dye, a cell, an antibody, enzymes, peroxidase, lipase, amylose, organophosphate dehydrogenase, ligases, restriction endonucleases, ribonucleases, DNA polymerases, glucose oxidase, laccase, cells, viruses, proteins, peptides, small molecules, drugs, dyes, amino acids, vitamins, antixoxidants, DNA, RNA, RNAi, lipids, nucleotides, aptamers, carbohydrates, chromophores, light emitting organic compounds, luciferin, carotenes and light emitting inorganic compounds, antibiotics, antifungals, antivirals, light harvesting compounds, bacteriorhodopsin, protorhodopsin, porphyrins, electronically active compounds, or combinations thereof.

7. The device of claim 1 , wherein the at least one channel is a microchannel.

8. A method of forming the device of claim 1 , comprising steps of:
providing a substrate;
casting a silk fibroin solution on the substrate;
embedding a channel mold in the silk fibroin solution;
solidify said silk fibroin solution; and
extracting the embedded channel mold thereby forming the device made of the solidified silk fibroin matrix.

9. The method of claim 8 , wherein the silk fibroin solution contains approximately 1.0 wt % to 30 wt % silk fibroin.

10. The device of claim 3 , wherein the patterned features on the surface of the device have a localized surface roughness below 20 nm.

11. The device of claim 1 , wherein a surface of the device comprises features patterned to form a microlens array characterized in that when the device exposed to incident light, the microlens array localizes the light below the surface of the device, and wherein the embedded organic material is or comprises light harvesting compounds that collect light and store energy.

Assignees

• Univ Tufts

Inventors

• Omenetto Fiorenzo
• Kaplan David L
• Lawrence Brian
• Cronin-Golomb Mark

Classifications

• G02B5/18

  Diffraction gratings {(holographic optical elements G02B5/32, G03H; integrally combined with optical fibres G02B6/02057; for coupling light guides G02B6/34; integrally combined with optical integrated light guides G02B6/12; grating systems G02B27/44)} · CPC title

• D10B2401/20

  optical · CPC title

• D10B2211/04

  Silk · CPC title

• G02B2006/12102

  Lens · CPC title

• G01N33/54373

  involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings · CPC title