Biological tissue cutting device and use thereof

Patent metadata

Field	Value
Publication number	US-10036689-B2
Application number	US-201515115491-A
Country	US
Kind code	B2
Filing date	Jan 27, 2015
Priority date	Jan 29, 2014
Publication date	Jul 31, 2018
Grant date	Jul 31, 2018

Abstract

The present invention provides a biological tissue cutting device that can produce minute fragments of a biological tissue easily and efficiently. The biological tissue cutting device for producing tissue fragments by cutting a biological tissue includes: a first cutting unit configured to form a cut surface extending in a first direction by cutting the biological tissue on a stage; and a second cutting unit configured to form a cut surface extending in a second direction and a cut surface extending in a third direction by cutting the biological tissue on the stage. The planar direction of the cut surface extending in the second direction is a direction crossing the cut surface extending in the first direction, and the planar direction of the cut surface extending in the third direction is a direction crossing the cut surface extending in the first direction and the cut surface extending in the second direction.

First claim

The invention claimed is:

1. A biological tissue cutting device for producing a tissue fragment by cutting a biological tissue, the biological tissue cutting device comprising:
a stage unit comprising a stage configured to fix the biological tissue;
a first cutting unit configured to form a cut surface extending in a first direction by cutting the biological tissue fixed on the stage; and
a second cutting unit configured to form a cut surface extending in a second direction and a cut surface extending in a third direction by cutting the biological tissue fixed on the stage, wherein
a planar direction of the cut surface extending in the second direction is a direction crossing the cut surface extending in the first direction,
a planar direction of the cut surface extending in the third direction is a direction crossing the cut surface extending in the first direction and the cut surface extending in the second direction,
the second cutting unit comprises:
a second cutting section that cuts the biological tissue to form the cut surface extending in the second direction;
a third cutting section that cuts the biological tissue to form the cut surface extending in the third direction; and
a second movement control section that moves the second cutting section and the third cutting section simultaneously,

the second cutting section and the third cutting section are integrated in such a manner that the cut surface extending in the second direction and the cut surface extending in the third direction formed by the second cutting section and the third cutting section maintain a fixed angle, and
the second movement control section simultaneously moves the second cutting section and the third cutting section from a cutting start position to a cutting end position of the biological tissue and then to a new cutting start position.

2. The biological tissue cutting device according to claim 1 , wherein
the first cutting unit comprises:
a first cutting section that cuts the biological tissue to form the cut surface extending in the first direction; and
a first movement control section that moves the first cutting section, and

the first movement control section moves the first cutting section from a cutting start position to a cutting end position of the biological tissue and then to a new cutting start position.

3. The biological tissue cutting device according to claim 2 , wherein
after the first movement control section has moved the first cutting section from the cutting start position to the cutting end position, the first movement control section moves the first cutting section away from the biological tissue and then to the new cutting start position.

4. The biological tissue cutting device according to claim 1 , wherein
after the second movement control section has moved the second cutting section and the third cutting section from the cutting start position to the cutting end position of the biological tissue, the second movement control section moves the second cutting section and the third cutting section from the cutting end position to the cutting start position along the path, and further moves the second cutting section and the third cutting section to a new cutting start position.

5. The biological tissue cutting device according to claim 1 , further comprising a stage movement control section, wherein
the stage movement control section moves the stage in at least one of an upper direction, a lower direction, and a planar direction relative to a surface of the stage on which the biological tissue is fixed.

6. The biological tissue cutting device according to claim 1 , wherein
the first cutting unit and the second cutting unit are provided as separate components,
each of the first cutting unit and the second cutting unit comprises a stage mounting section,
the stage is mounted to the stage mounting section of the first cutting unit and the first cutting unit perform cutting of the biological tissue, and thereafter, the stage is detached from the first cutting unit and mounted to the second cutting unit and the second cutting unit performs cutting of the biological tissue.

7. The biological tissue cutting device according to claim 1 , wherein
the first cutting unit and the second cutting unit are disposed in a single component, and
at least one of the stage, the first cutting unit, and the second cutting unit is detachable.

8. A method for cutting a biological tissue using the biological tissue cutting device according to claim 1 , the method comprising:
a fixing step of fixing a biological tissue on the stage;
a first cutting step of cutting the biological tissue by the first cutting unit to form a cut surface extending in a first direction; and
a second cutting step of cutting the biological tissue by the second cutting unit to form a cut surface extending in a second direction and a cut surface extending in a third direction, wherein
a planar direction of the cut surface extending in the second direction is a direction crossing the cut surface extending in the first direction, and
a planar direction of the cut surface extending in the third direction is a direction crossing the cut surface extending in the first direction and the cut surface extending in the second direction.

9. The method according to claim 8 , wherein
the biological tissue is at least one selected from the group consisting of cartilage, membrane tissues, skin tissues, vascular tissues, hormone-producing tissues,
nerves, tendons, liver tissues, fat, and muscles.

10. The method according to claim 8 , wherein
the biological tissue fixed on the stage is from 0.1 to 10 mm in thickness and from 50 to 10,000 mm 2 in area.

11. The method according to claim 8 , wherein
the length of each side of a fragment of the biological tissue obtained through the cutting by the first cutting unit and the second cutting unit is in a range from 0.05 to 3 mm.

12. The method according to claim 8 , wherein
the cut surface of the biological tissue in the first direction is substantially perpendicular to the surface of the stage, and the cut surface extending in the second direction of the biological tissue is substantially perpendicular to a surface of the stage,
the cut surface extending in the first direction is substantially perpendicular to the cut surface extending in the second direction, and
the cut surface of the biological tissue in the third direction is substantially parallel to the surface of the stage.

13. The method according to claim 8 , wherein
the first cutting step and the second cutting step are repeated for the same biological tissue.

14. A method for producing a tissue fragment, the method comprising:
cutting a biological tissue by the method according to claim 8 .

Assignees

• Jms Co Ltd

Inventors

• Shibata Kazuhiro
• Isogai Noritaka
• Suzuki Koji

Classifications

• A61P43/00

  Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title

• G01N1/06

  providing a thin slice, e.g. microtome · CPC title

• G01N2001/2873

  Cutting or cleaving · CPC title

• A61B17/32

  Surgical cutting instruments ({A61B18/042 takes precedence; suture cutters A61B17/0467;} implements for ligaturing and cutting {A61B17/122, A61B17/12; instruments for rupturing the amniotic membrane A61B17/4208; specially adapted knives for eye surgery A61F9/0133}) · CPC title

• G01N1/286 Primary

  involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising (microtomes G01N1/06; pulverising in general B02C; mixing in general B01F) · CPC title