Slide chip for detection sensor of food-borne pathogens and preparation method thereof

Patent metadata

Field	Value
Publication number	US-10036073-B2
Application number	US-201214343213-A
Country	US
Kind code	B2
Filing date	Feb 22, 2012
Priority date	Aug 4, 2011
Publication date	Jul 31, 2018
Grant date	Jul 31, 2018

Abstract

The present invention relates to a slide chip for a sensor for detection of food-borne bacteria and a fabrication method thereof. More particularly, the invention relates to a slide chip for a sensor for detection of food-borne bacteria and a fabrication method thereof, the slide chip comprising: a substrate coated with a metal; a linker having a substituent which may be bonded to the metal and is located at the 5′ end of deoxythymidine (dT); and a food-borne bacterium-derived RNA aptamer that is bound to the linker by the 3′-end poly A tail. The slide chip makes it possible to detect food-borne bacteria in a rapid and accurate manner.

First claim

The invention claimed is:

1. A slide glass for an optical microscope for detection of food-borne bacteria comprising:
a glass substrate nano-coated with a metal;
a linker having a substituent bonded to the metal and located at the 5′ end of deoxythymidine (dT); and
an RNA aptamer bonded to the linker by the 3′-end poly A tail,
wherein the RNA aptamer is an aptamer binding to lipopolysaccharide of E. coli O157:H7, an aptamer binding to ompC protein of Salmonella typhimurium strain, or an aptamer binding to teichoic acid of Staphylococcus aureus.

2. The slide glass of claim 1 , wherein the RNA aptamer is obtained by substituting the 2′ carbon of RNA pyrimidine (cytosine and uracil) with fluorine.

3. The slide glass of claim 1 , wherein the metal is silver or gold.

4. The slide glass of claim 1 , wherein the substituent is a thiol group (—SH).

5. The slide chip of claim 1 , wherein the slide glass further comprises a fluorescence tag having a 6-FAM molecule for fluorescence labeling of food-borne bacteria bonded to the RNA aptamer, the fluorescence tag is being located at the 5′ end of deoxythymidine (dT).

Assignees

• Cho Young Jin
• Kim Chul Jin
• Kim Nam Soo
• Kim Chong Tai
• Maeng Jin Soo
• Kim Tae Eun
• Lee Myung Hyun
• Korea Food Res Inst

Inventors

• Cho Young Jin
• Kim Chul Jin
• Kim Nam Soo
• Kim Chong Tai
• Maeng Jin Soo
• Kim Tae Eun
• Lee Myung Hyun

Classifications

• G01N33/5017

  for testing neoplastic activity · CPC title

• C12N15/111

  General methods applicable to biologically active non-coding nucleic acids · CPC title

• G01N33/56938

  Staphylococcus · CPC title

• C12Q1/689 Primary

  for bacteria · CPC title

• G01N33/54366

  Apparatus specially adapted for solid-phase testing · CPC title