Sequences involved in plant yield and methods of using

Patent metadata

Field	Value
Publication number	US-10036034-B2
Application number	US-201414562182-A
Country	US
Kind code	B2
Filing date	Dec 5, 2014
Priority date	Feb 13, 2014
Publication date	Jul 31, 2018
Grant date	Jul 31, 2018

Abstract

Nucleic acid sequences involved in plant yield are provided, as are methods of using such nucleic acid sequences.

First claim

What is claimed is:

1. A plant variety, line, or cultivar comprising plants having a mutation in one or more endogenous nucleic acids having a sequence selected from the group consisting of SEQ ID NOs: 1 and 3, wherein the mutation results in reduced expression of the endogenous nucleic acid relative to corresponding plants lacking the mutation, wherein the plants exhibit an increase in the length of the primary root under limiting water conditions, an increase in the length of the seminal root under limiting water conditions, an increase in lateral root density under limiting water conditions, an increase in root biomass under limiting water conditions, an increase in the number of seeds per plant under water conditions that are not limiting, an increase in the average size of the seed under water conditions that are not limiting, and/or an increase in the average weight of the seed under water conditions that are not limiting relative to a corresponding plant lacking the mutation under corresponding conditions.

2. Seed produced by the plant variety, line, or cultivar of claim 1 , the seed comprising the mutation in one or more endogenous nucleic acids having a sequence selected from the group consisting of SEQ ID NOs: 1 and 3, wherein the mutation results in reduced expression of the endogenous nucleic acid in plants grown from the seed, relative to corresponding plants lacking the mutation, wherein plants grown from the seed exhibit an increase in the length of the primary root under limiting water conditions, an increase in the length of the seminal root under limiting water conditions, an increase in lateral root density under limiting water conditions, an increase in root biomass under limiting water conditions, an increase in the number of seeds per plant under water conditions that are not limiting, an increase in the average size of the seed under water conditions that are not limiting, and/or an increase in the average weight of the seed under water conditions that are not limiting relative to a plant grown from corresponding seed lacking the mutation grown under corresponding conditions.

3. A method of making a plant, comprising the steps of:
inducing mutagenesis in plant cells to produce mutagenized cells;
obtaining one or more plants from the mutagenized cells;
identifying plants that comprises a mutation in one or more endogenous nucleic acids having a sequence selected from the group consisting of SEQ ID NOs: 1 and 3 and exhibit reduced expression of the endogenous nucleic acid relative to plants lacking the mutation; and
identifying plants that exhibits an increase in the length of the primary root under limiting water conditions, an increase in the length of the seminal root under limiting water conditions, an increase in lateral root density under limiting water conditions, an increase in root biomass under limiting water conditions, an increase in the number of seeds per plant under water conditions that are not limiting, an increase in the average size of the seed under water conditions that are not limiting, and/or an increase in the average weight of the seed under water conditions that are not limiting relative to a corresponding plant lacking the mutation under corresponding conditions.

4. The method of claim 3 , wherein mutagenesis is induced using a chemical mutagen or ionizing radiation.

5. The method of claim 4 , wherein the chemical mutagen is selected from the group consisting of nitrous acid, sodium azide, acridine orange, ethidium bromide, and ethyl methane sulfonate (EMS).

6. The method of claim 4 , wherein the ionizing radiation is selected from the group consisting of x-rays, gamma rays, fast neutron irradiation, and UV irradiation.

7. The method of claim 3 , wherein mutagenesis is induced using TALEN technology, zinc-finger technology, or CRISPR technology.

8. A method for producing a plant, said method comprising the steps of:
crossing at least one plant of a first line with at least one plant of a second line, the plant of the first line having a mutation in one or more endogenous nucleic acids having a sequence selected from the group consisting of SEQ ID NOs: 1 and 3, wherein the mutation results in reduced expression of the endogenous nucleic acid relative to a corresponding plant lacking the mutation; and
selecting for progeny plants that have the mutation and exhibit reduced expression of the endogenous nucleic acid relative to a corresponding plant lacking the mutation; and
selecting for progeny plants exhibiting an increase in the length of the primary root under limiting water conditions, an increase in the length of the seminal root under limiting water conditions, an increase in lateral root density under limiting water conditions, an increase in root biomass under limiting water conditions, an increase in the number of seeds per plant under water conditions that are not limiting, an increase in the average size of the seed under water conditions that are not limiting, and/or an increase in the average weight of the seed under water conditions that are not limiting relative to a corresponding plant lacking the mutation under corresponding conditions.

9. A food or feed product comprising plant material from the plant of claim 1 .

10. The food or feed product of claim 9 , wherein the food product is for human consumption.

11. The food or feed product of claim 9 , wherein the feed product is for animal consumption.

12. A method of producing a food or feed product, the method comprising:
providing plant material from a plant having a mutation in one or more endogenous nucleic acids having a sequence selected from the group consisting of SEQ ID NOs: 1 and 3, wherein the mutation results in reduced expression of the endogenous nucleic acid relative to a plant lacking the mutation, wherein the plants exhibit an increase in the length of the primary root under limiting water conditions, an increase in the length of the seminal root under limiting water conditions, an increase in lateral root density under limiting water conditions, an increase in root biomass under limiting water conditions, an increase in the number of seeds per plant under water conditions that are not limiting, an increase in the average size of the seed under water conditions that are not limiting, and/or an increase in the average weight of the seed under water conditions that are not limiting relative to a corresponding plant lacking the mutation under corresponding conditions; and
manufacturing a food or feed product using the plant material.

13. The method of claim 12 , wherein the mutation is selected from the group consisting of a point mutation, an insertion, a deletion, and a substitution.

Assignees

• Nutech Ventures

Inventors

• Walia Harkamal
• Placido Dante
• Clemente Thomas E

Classifications

• C12N15/8261

  with agronomic (input) traits, e.g. crop yield · CPC title

• C12N15/8273 Primary

  for drought, cold, salt resistance · CPC title

• C07K14/415

  from plants · CPC title

• C12Q1/6895

  for plants, fungi or algae · CPC title

• A23L7/198

  Dry unshaped finely divided cereal products, not provided for in groups A23L7/117 - A23L7/196 and A23L29/00, e.g. meal, flour, powder, dried cereal creams or extracts · CPC title