Cell washing device using a wave

Patent metadata

Field	Value
Publication number	US-10036006-B2
Application number	US-201414519284-A
Country	US
Kind code	B2
Filing date	Oct 21, 2014
Priority date	Oct 21, 2013
Publication date	Jul 31, 2018
Grant date	Jul 31, 2018

Abstract

A system is disclosed for washing a selected component and removing the selected component from a wash material. The selected component may include red blood cells that are washed to remove a rejuvenating solution. The red blood cells may then be removed with various systems, such as a standing acoustic wave system from the wash solution. Pumps and flow restrictors that provide steady flow from pumps that generate pulsed flow are also disclosed.

First claim

What is claimed is:

1. A method of separating a component from a wash material, comprising:
mixing the wash material with a sample of the component;
transferring the wash material and component, after mixing, to a separating system including a mixing section;
separating the component from the wash material, wherein separating the component includes injecting a directing flow into the mixing section to direct the component towards a collection chamber; and
collecting the component in the collection chamber.

2. The method of claim 1 , wherein the component includes red blood cells.

3. The method of claim 2 , wherein separating the component includes separating red blood cells from the wash material with a standing wave.

4. The method of claim 3 , further comprising generating the standing wave as a standing acoustic wave.

5. The method of claim 4 , wherein the standing acoustic wave is generated on a surface.

6. The method of claim 1 , wherein collecting the component occurs after separating the component from the wash material.

7. A method of separating a component from a wash material, comprising:
mixing the wash material with a sample comprising the component to generate a mixture;
transferring the mixture to a separating system comprising a collection chamber, a first wave generator, and a second wave generator, wherein the first and second wave generators generate a standing surface acoustic wave;
separating the component from the mixture in the separation system, wherein separating the component includes injecting a directing flow into the mixing section to direct the component towards the collection chamber; and
collecting the component in the collection chamber.

8. The method of claim 7 , wherein mixing comprises flowing the sample from a sample source to a mixing chamber, and flowing the wash material from a wash material source to the mixing chamber, wherein the sample and the wash material are mixed to generate the mixture.

9. The method of claim 7 , wherein the transferring the mixture to the separating system comprises pumping the mixture into the separating system with a pump.

10. The method of claim 7 , wherein separating the component from the mixture comprises flowing the mixture through a flow channel relative to the standing surface acoustic wave, wherein the standing surface acoustic moves the component to a component collection path.

11. The method of claim 10 , wherein separating the component from the mixture further comprises flowing the wash material through a wash material collection path, wherein the wash material collection path is different from the component collection path.

12. The method of claim 7 , wherein mixing the wash material with a sample comprising the component includes mixing the wash material with a rejuvenated blood sample, wherein the component comprises red blood cells.

13. A method of separating red blood cells from a wash material, comprising:
transferring a sample of red blood cells and a wash material into a mixing chamber;
mixing the red blood cells with the wash material within the mixing chamber to form a mixture;
pumping the mixture from the mixing chamber to a separation system comprising a flow channel, a first wave generator and a second wave generator, wherein the first and second wave generators generate a standing surface acoustic wave relative to the flow channel;
separating the red blood cells from the wash material by flowing the mixture through the flow channel relative the standing surface acoustic wave and injecting a directing flow into the flow channel to direct the red blood cells towards a red blood cell collection path; and
collecting the red blood cells from the red blood cell collection path.

14. The method of claim 13 , further comprising rejuvenating red blood cells by mixing red blood cells with a rejuvenating solution to generate the sample of red blood cells.

15. The method according to claim 14 , wherein separating further comprises separating the red blood cells from the rejuvenating solution.

16. The method according to claim 13 , wherein mixing comprises mixing the red blood cells and wash material at about 30° C. to about 36° C. for about 2 minutes to about 10 minutes.

17. The method according to claim 13 , further comprising infusing the red blood cells to a patient in need thereof.

18. A system for separating a component from a mixture, comprising:
a mixing chamber; and
a separation apparatus comprising a flow channel positioned relative to a first wave generator and a second wave generator, the first and second wave generators configured to generate a standing surface acoustic wave relative to the flow channel, the flow channel defining a plurality of inlet ports, each of the plurality of inlet ports arranged to inject a directing flow into a flow of the mixture passing through the flow channel,
wherein the mixing chamber is in fluid communication with the separation apparatus.

19. The system according to claim 18 , further comprising a source of a sample and a source of a wash material; wherein the source of a sample and the source of a wash material are in fluid communication with the mixing chamber.

20. The system according to claim 18 , wherein the flow channel comprises a cross-section area of about 0.1 μm 2 to about 100 cm 2 .

21. The system according to claim 18 , wherein the mixing chamber is in communication with the separation apparatus by a conduit that couples the mixing chamber to a first portion of the separation apparatus.

22. The system according to claim 21 , wherein the separation apparatus further comprises a second portion opposite the first portion, wherein the flow channel is bifurcated into a component path and a wash solution path near the second portion, the component path being larger than the wash solution path.

23. The system according to claim 22 , wherein the wash solution path has a diameter about 10% to about 2000% larger than a diameter of the component path.

Assignees

• Biomet Biologics Llc

Inventors

• Leach Michael D

Classifications

• A61M1/0281

  Apparatus for treatment of blood or blood constituents prior to transfusion, e.g. washing, filtering or thawing · CPC title

• G01N2015/0288

  Sorting the particles · CPC title

• A61K35/18

  Erythrocytes (haemoglobin A61K38/42) · CPC title

• B01L2400/0433

  vibrational forces · CPC title

• B01D21/283 Primary

  Settling tanks provided with vibrators · CPC title