Parallelized sample processing and library prep
US-12097501-B2 · Sep 24, 2024 · US
US10035149B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10035149-B2 |
| Application number | US-201514967788-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 14, 2015 |
| Priority date | Dec 17, 2014 |
| Publication date | Jul 31, 2018 |
| Grant date | Jul 31, 2018 |
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A mixing container includes a first container that stores two or more reagents and a second container for mixing two or more reagents. The first container includes reagent storage chambers that are respectively provided for the two or more reagents and have openings formed at portions thereof, and a sealing member that seals the respective openings of the reagent storage chambers. The second container includes an opening portion formed such that the first container can be connected thereto, and protruding portions disposed so as to pierce the sealing member when the first container is connected to the opening portion.
Opening claim text (preview).
What is claimed is: 1. A mixing container for mixing two or more reagents, comprising: a first container storing two or more reagents; and a second container for mixing the two or more reagents, wherein the first container includes: reagent storage chambers that are respectively provided for the two or more reagents and have openings formed at portions thereof, the reagent storage chambers extending from a bottom surface of the first container and beyond a side portion of the first container; and a sealing member that seals respective openings of the reagent storage chambers, and the second container includes: an opening portion formed so that the first container can be connected thereto; and protruding portions disposed so as to pierce the sealing member when the first container is connected to the opening portion, the protruding portions being positioned below an upper surface of the second container and being aligned within the second container, wherein, the protruding portions provided with a plurality of first edges are formed to continue along a plurality of second edges of the openings of the reagent storage chambers, and the protruding portions are sized to fit inside the reagent storage chambers, while a cut part of the protruding portions is formed to be cut along the second edges of the openings of the reagent storage chambers, such that a substantial portion of the perimeter of the sealing member along the second edges of the openings of the reagent storage chambers are cut, while a part of the portions of the sealing member are not cut by the protruding portions, when the first container is connected to the opening portion of the second container. 2. The mixing container according to claim 1 , wherein in the first container, the reagent storage chambers are formed such that the openings face an identical direction, and in the second container, the opening portion is formed such that the first container can be connected thereto by fitting with the sealing member facing the second container, and the number of the protruding portions is the same as the number of the reagent storage chambers, and the protruding portions are disposed so as to face the respective corresponding storage chambers. 3. The mixing container according to claim 1 , wherein the first container includes a through hole, and the through hole is formed such that an interior and exterior of the second container are in communication when the first container is connected to the second container. 4. The mixing container according to claim 3 , comprising a nozzle that can drip a liquid and is formed to be attachable to the through hole. 5. The mixing container according to claim 1 , wherein the protruding portions each include a shearing portion and a pushing portion, the shearing portion is formed so as to protrude more toward the sealing member than the pushing portion does, and the pushing portion is formed at a position closer to a portion of the sealing member that is not to be cut than the shearing portion is. 6. The mixing container according to claim 3 , wherein an inclined surface is provided below the protruding portions inside of the second container.
pierceable, e.g. films, membranes · CPC title
Sealing · CPC title
with fluid transport, e.g. in multi-compartment structures · CPC title
Align devices or objects to ensure defined positions relative to each other · CPC title
Integrated piercing tool · CPC title
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