Microorganism comprising gene for coding enzyme involved in producing retinoid and method for producing retinoid by using same

Patent metadata

Field	Value
Publication number	US-10030258-B2
Application number	US-201314900303-A
Country	US
Kind code	B2
Filing date	Oct 28, 2013
Priority date	Jun 20, 2013
Publication date	Jul 24, 2018
Grant date	Jul 24, 2018

Abstract

The present invention relates to a microorganism comprising a gene for coding an enzyme involved in producing retinoid and a method for producing retinoid by using the same, and more specifically, to: a microorganism capable of mass-producing retinoid at a remarkable efficiency by comprising a gene for coding an enzyme involved in producing retinoid; and a method for producing retinoid by using the same.

First claim

The invention claimed is:

1. A microorganism of the genus Saccharomyces , comprising genes coding enzymes involved in retinoid production.

2. The microorganism according to claim 1 , wherein the genes code at least one amino acid sequence selected from the group consisting of SEQ ID NOs 1 to 9.

3. The microorganism according to claim 1 , wherein the genes code an amino acid sequence of at least one of SEQ ID NOs 2, 3 and 10; at least one of SEQ ID NOs 4 and 11; at least one of SEQ ID NOs 5, 6 and 12; SEQ ID NO 7; SEQ ID NO 8; and at least one of SEQ ID NOs 9, 13 and 21.

4. The microorganism according to claim 3 , further comprising a gene coding an amino acid sequence of SEQ ID NO 1.

5. The microorganism according to claim 1 , wherein the microorganism is Saccharomyces cerevisiae.

6. The microorganism according to claim 1 , wherein the microorganism is Saccharomyces cerevisiae Y2805.

7. A microorganism (the genus Corynebacterium ) comprising genes coding enzymes involved in retinoid production.

8. The microorganism according to claim 7 , wherein the genes code at least one amino acid sequence selected from the group consisting of SEQ ID NOs 2 to 9.

9. The microorganism according to claim 7 , wherein the genes code an amino acid sequence of at least one of SEQ ID NOs 2, 3 and 10; at least one of SEQ ID NOs 4 and 11; at least one of SEQ ID NOs 5, 6 and 12; SEQ ID NO 7; SEQ ID NO 8; and at least one of SEQ ID NOs 9, 13 and 21.

10. The microorganism according to claim 9 , further comprising a gene coding at least one amino acid sequence of SEQ ID NOs 14 and 15.

11. The microorganism according to claim 9 , further comprising a gene coding an amino acid sequence of SEQ ID NOs 16 to 20.

12. The microorganism according to claim 7 , wherein, in the microorganism, a gene coding at least one amino acid sequence selected from the group consisting of SEQ ID NOs 22 to 24 is inactivated or deleted.

13. The microorganism according to claim 7 , wherein the microorganism is Corynebacterium glutamicum.

14. The microorganism according to claim 7 , wherein the microorganism is Corynebacterium glutamicum ATCC13032.

15. The microorganism according to claim 1 , wherein the gene is introduced by a vector.

16. The microorganism of claim 1 , wherein the microorganism is transformed with a gene encoding a hydroxymethylglutaryl(HMG)-CoA reductase among enzymes in a MVA pathway, and the microorganism has retinoid producing efficacy without being transformed with genes encoding acetyl-CoA acetyltransferase, HMG-CoA synthase, mevalonate kinase, phosphomevalonate kinase, diphosphomevalonate decarboxylase, or isopentenyl diphosphate isomerase in an MVA pathway.

Assignees

• Nat Univ Gyeongsang Iacf
• Industrial Academic Cooperation Foundation Gyeongsang National Univ

Inventors

• Kim Seon-Won
• Jang Hui-Jeong
• Lee Hyeon-Seo
• Yoon Sang-Hwal

Classifications

• C12P23/00 Primary

  Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes (containing heterorings C12P17/00) · CPC title

• C12R1/865

  Chemistry & Metallurgy · mapped topic

• C12R1/15

  Chemistry & Metallurgy · mapped topic

• C12N1/18 Primary

  Baker's yeast; Brewer's yeast · CPC title

• C12P1/00

  Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes · CPC title