Method for producing lysophosphatidylethanolamine 18:1 from microorganism of Pseudomonas sp

Patent metadata

Field	Value
Publication number	US-10030256-B2
Application number	US-201715852864-A
Country	US
Kind code	B2
Filing date	Dec 22, 2017
Priority date	Jun 24, 2014
Publication date	Jul 24, 2018
Grant date	Jul 24, 2018

Abstract

A method for producing lysophosphatidylethanolamine 18:1 includes extracting phospholipids including mainly phosphatidylethanolamine from a microorganism of Pseudomonas sp. and treating the extracted phospholipids with phospholipase A2. An alternative method for producing lysophosphatidylethanolamine 18:1 includes treating a microorganism of Pseudomonas sp. directly with phospholipase A2. The lysophosphatidylethanolamine 18:1 can be used as a plant vaccine material for preventing the plants from injuries caused by pathogen infections and/or environmental stresses and accelerating the recovery of plants injured by pathogen infections and/or environmental stresses, and can also be used as a composition for enhancing fruit ripening (color and sweetness) and storage properties, and as it can be used for an application in plant tissues, food products, pharmaceuticals, cosmetics, and agricultural use, it would be very advantageously used in related industries. This invention also provides a method of producing a phosphatidylethanolamine itself from a microorganism of Pseudomonas sp.

First claim

What is claimed is:

1. A method for production of lysophosphatidylethanolamine comprising:
performing culture of a microorganism of Pseudomonas sp.;
obtaining the microorganism from the culture;
extracting phospholipid comprising phosphatidylethanolamine from the obtained microorganism;
treating the extracted phospholipid comprising the phosphatidylethanolamine with phospholipase A 2 to produce lysophosphatidylethanolamine; and
separating and purifying the produced lysophosphatidylethanolamine.

2. The method of claim 1 , wherein the lysophosphatidylethanolamine has lysophosphatidylethanolamine 18:1.

3. The method of claim 1 , wherein the microorganism of Pseudomonas sp. is Pseudomonas caeni, Pseudomonas fluorescens, Pseudomonas putida , or Pseudomonas syringae.

4. The method of claim 1 , wherein the phospholipid is the phosphatidylethanolamine.

5. The method of claim 4 , wherein the phosphatidylethanolamine has an 18:1 acyl chain at the first position.

6. The method of claim 1 , wherein the phospholipase A 2 originates from an animal, a microorganism, or a plant.

Assignees

• Korea Res Inst Bioscience & Biotechnology

Inventors

• Ryu Beung Tae
• Lee Young Pyo
• Jung Ji Hye
• Jung Sung Hee

Classifications

• C07F9/103

  Extraction or purification by physical or chemical treatment of natural phosphatides; Preparation of compositions containing phosphatides of unknown structure · CPC title

• C12P13/001 Primary

  Amines; Imines · CPC title

• C12Y301/01004

  Phospholipase A2 (3.1.1.4) · CPC title

• C07F9/10

  Phosphatides, e.g. lecithin · CPC title

• C12P9/00 Primary

  Preparation of organic compounds containing a metal or atom other than H, N, C, O, S or halogen {(phosphoglycerides, C12P7/6481)} · CPC title