Transgenic plant of the species solanum tuberosum with resistance to phytophthora

Patent metadata

Field	Value
Publication number	US-10030251-B2
Application number	US-201314420106-A
Country	US
Kind code	B2
Filing date	Aug 6, 2013
Priority date	Aug 8, 2012
Publication date	Jul 24, 2018
Grant date	Jul 24, 2018

Abstract

The present invention concerns a transgenic plant of the species Solanum tuberosum with a resistance to an oomycete of the genus Phytophthora, transgenic parts of such a plant, a method for its manufacture and to a composition for external application to plants. On the one hand, nucleotide sequences in accordance with SEQ ID NOS: 1-43 are provided from Phytophthora in a host plant-induced gene silencing strategy in potato plants; on the other hand, a fungicide for plant treatment is provided.

First claim

The invention claimed is:

1. A transgenic plant of the species Solanum tuberosum or a part thereof comprising a stably integrated first double-stranded DNA and a stably integrated second double-stranded DNA, wherein the nucleotide sequences of the coding strands of the first and second DNA are reverse complements of each other, so that a transcript of the first DNA and a transcript of the second DNA hybridize to form a double-stranded RNA and whereby the plant expressing said double-stranded RNA is resistant to an oomycete of the genus Phytophthora , wherein the coding strand of the first DNA comprises:
(a) at least 23 successive nucleotides of the nucleotide sequence of SEQ ID NO: 1, or
(b) a nucleotide sequence which is the complementary sequence to at least 23 successive nucleotides of the nucleotide sequence of SEQ ID NO: 1.

2. The transgenic plant or a part thereof of claim 1 , wherein the coding strand of the first DNA comprises:
(a) the nucleotide sequence of SEQ ID NO: 1, or
(b) a nucleotide sequence which is the complementary sequence to the nucleotide sequence of SEQ ID NO: 1.

3. The transgenic plant or a part thereof of claim 1 , wherein the plant or a part thereof exhibits a resistance to Phytophthora infestans.

4. The transgenic plant or a part thereof of claim 1 , wherein the double-stranded RNA is miRNA or siRNA.

5. The transgenic plant or a part thereof of claim 1 , wherein the first DNA and the second DNA are operatively linked to at least one promoter.

6. The transgenic plant or a part thereof of claim 1 , wherein the first DNA and the second DNA are under the control of the same promoter and are separated by an intron.

7. The part of the transgenic plant of claim 1 , wherein the part is a seed or a cell.

8. The transgenic plant or a part thereof of claim 1 , wherein the first and second DNA are complete reverse complements of each other.

9. The transgenic plant or a part thereof of claim 1 , wherein the first and second DNA are partial reverse complements of each other.

10. A method for producing the transgenic plant of claim 1 , which plant exhibits a resistance to an oomycete of the genus Phytophthora , comprising the following steps:
(i) producing a transformed first parent plant comprising a first double-stranded DNA which is stably integrated into the genome of said first parent plant and which has a coding strand which comprises:
(a) at least 23 successive nucleotides of the nucleotide sequence of SEQ ID NO: 1, or
a nucleotide sequence which is the complementary sequence to at least 23 successive nucleotides of the nucleotide sequence of SEQ ID NO: 1;

(ii) producing a transformed second parent plant comprising a second double-stranded DNA which is stably integrated into the genome of said second parent plant, wherein the nucleotide sequences of the coding strands of the first and second DNA are reverse complements of each other, so that a transcript of the first DNA and a transcript of the second DNA hybridize to form a double-stranded RNA and whereby the plant expressing said double-stranded RNA becomes resistant to an oomycete of the genus Phytophthora;
(iii) crossing the first parent plant with the second parent plant;
(iv) selecting a plant in the genome of which both the first double-stranded DNA and the second double-stranded DNA have been stably integrated.

11. The method of claim 10 , wherein the coding strand of the first DNA comprises:
(a) the nucleotide sequence of SEQ ID NO: 1, or
(b) a nucleotide sequence which is the complementary sequence to the nucleotide sequence of SEQ ID NO: 1.

12. The method of claim 10 , wherein the resistance is resistance to Phytophthora infestans.

13. The method of claim 10 , wherein the double-stranded RNA is miRNA or siRNA.

14. The method of claim 10 , wherein the first and second DNA are complete reverse complements of each other.

15. The method of claim 10 , wherein the first and second DNA are complete reverse complements of each other.

Assignees

• Kws Saat Ag
• Kws Saat Se & Co Kgaa

Inventors

• Stahl Jurgen Dietmar
• Temme Nora

Classifications

• C12N15/8282 Primary

  for fungal resistance · CPC title

• C12N2310/141

  MicroRNAs, miRNAs · CPC title

• C12N2310/14

  interfering nucleic acids [NA] · CPC title

• C12N15/113

  Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; {Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing (when used in plants C12N15/8218)} · CPC title